Current approaches for RNA-labelling to identify RNA-binding proteins.

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  • Additional Information
    • Source:
      Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 8606068 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1208-6002 (Electronic) Linking ISSN: 08298211 NLM ISO Abbreviation: Biochem Cell Biol Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : Ottawa, ON : Canadian Science Publishing
      Original Publication: Ottawa, Ont. : National Research Council of Canada, c1986-
    • Subject Terms:
      RNA/*analysis ; RNA/*chemistry ; RNA-Binding Proteins/*analysis ; RNA-Binding Proteins/*chemistry; Biotin/chemistry ; Humans ; Molecular Structure ; Small Molecule Libraries/chemistry
    • Abstract:
      RNA is involved in all domains of life, playing critical roles in a host of gene expression processes, host-defense mechanisms, cell proliferation, and diseases. A critical component in many of these events is the ability for RNA to interact with proteins. Over the past few decades, our understanding of such RNA-protein interactions and their importance has driven the search and development of new techniques for the identification of RNA-binding proteins. In determining which proteins bind to the RNA of interest, it is often useful to use the approach where the RNA molecule is the "bait" and allow it to capture proteins from a lysate or other relevant solution. Here, we review a collection of methods for modifying RNA to capture RNA-binding proteins. These include small-molecule modification, the addition of aptamers, DNA-anchoring, and nucleotide substitution. With each, we provide examples of their application, as well as highlight their advantages and potential challenges.
    • Contributed Indexing:
      Keywords: RNA-binding proteins; affinity capture; aptamer labelling; biotin labelling; capture d’affinité; digoxigenin labelling; méthode de substitution de nucléotides; nucleotide-substitution method; protéines liant l’ARN; étiquetage d’aptamères; étiquetage à la biotine; étiquetage à la digoxigénine
    • Accession Number:
      0 (RNA-Binding Proteins)
      0 (Small Molecule Libraries)
      63231-63-0 (RNA)
      6SO6U10H04 (Biotin)
    • Publication Date:
      Date Created: 20190402 Date Completed: 20200408 Latest Revision: 20200408
    • Publication Date:
      20231215
    • Accession Number:
      10.1139/bcb-2019-0041
    • Accession Number:
      30931575