Resistin up-regulates LPL expression through the PPARγ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264.7 macrophages.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9005353 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0023 (Electronic) Linking ISSN: 10434666 NLM ISO Abbreviation: Cytokine Subsets: MEDLINE
    • Publication Information:
      Publication: <2001- > : Oxford : Elsevier Science Ltd.
      Original Publication: [Philadelphia, PA] : Saunders Scientific Publications, W.B. Saunders, [c1989-
    • Subject Terms:
      Lipid Metabolism/*physiology ; Macrophages/*metabolism ; PPAR gamma/*metabolism ; Phosphatidylinositol 3-Kinase/*metabolism ; Proto-Oncogene Proteins c-akt/*metabolism ; Resistin/*metabolism ; Up-Regulation/*physiology; Animals ; Cell Line ; Mice ; RAW 264.7 Cells ; Signal Transduction/physiology ; Triglycerides/metabolism
    • Abstract:
      Resistin is a cysteine-rich cytokine, which has been indicated as a mediator of insulin resistance and inflammation. Previous studies demonstrated that lipoprotein lipase (LPL) was an important enzyme that could mediate lipid accumulation in macrophages. Additionally, the intracellular molecules phosphatidylinositol 3-kinase (PI3K)/serine-threonine protein kinase (AKT)/peroxisome proliferator-activated receptor (PPARγ) were supposed to be involved in the lipid accumulation process in cells. However, it remains unclear whether resistin was correlated with the dysregulation of lipid metabolism in macrophages. The present study investigated that resistin could up-regulate the expression of LPL and increase the contents of intracellular triglyceride (TG) and total cholesterol (TC) in RAW264.7 macrophages. In addition, intracellular molecules PI3K, AKT and PPARγ were significantly up-regulated and activated in resitin-stimulated RAW264.7 macrophages (P < 0.05). In contrast, the effects of resistin on RAW264.7 macrophages could be abrogated by specific inhibitors for LPL (LPL-siRNA) and PI3K/AKT signaling pathway (LY294002). All together, this study demonstrated that resistin could up-regulate the expression of LPL and induce lipid accumulation in RAW264.7 macrophages. More importantly, the PPARγ-dependent PI3K/AKT signaling pathway was relevant to the lipid accumulation process in resistin-stimulated macrophages.
      (Copyright © 2019 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Lipoprotein lipase; Macrophages; Peroxisome proliferator-activated receptor; Phosphatidylinositol 3-kinase; Resistin; Serine-threonine protein kinase
    • Accession Number:
      0 (PPAR gamma)
      0 (Resistin)
      0 (Triglycerides)
      EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
      EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
    • Publication Date:
      Date Created: 20190330 Date Completed: 20200806 Latest Revision: 20200806
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.cyto.2019.03.016
    • Accession Number:
      30925325