A predominant form of C-terminally end-cleaved AQP0 functions as an open water channel and an adhesion protein in AQP0 ^ΔC/ΔC mouse lens.

Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE

Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.

Aquaporins/*metabolism ; Eye Proteins/*metabolism ; Lens, Crystalline/*metabolism; Animals ; Aquaporins/chemistry ; Aquaporins/genetics ; Cataract/genetics ; Cataract/metabolism ; Cell Adhesion ; Cell Membrane Permeability ; Eye Proteins/chemistry ; Eye Proteins/genetics ; Lens, Crystalline/cytology ; Mice ; Mice, Inbred C57BL ; Mutation ; Water/metabolism

The purpose of this investigation was to find out whether C-terminally end-cleaved aquaporin 0 (AQP0), that is present predominantly in the lens mature fiber cells of the WT, functions as a water channel and a cell-to-cell adhesion (CTCA) protein in a knockin (KI) mouse model (AQP0 ^ΔC/ΔC ) that does not express intact AQP0. A genetically engineered KI mouse model, AQP0 ^ΔC/ΔC , expressing only end-cleaved AQP0 was developed. This model expresses 1-246 amino acids of AQP0, instead of the full length 1-263 amino acids. Lens transparency of postnatal day 10 (P10) was analyzed qualitatively by dark field imaging. WT, AQP0 ^{+/ -} and AQP0 ^+/ΔC lenses were transparent; AQP0 ^-/- and AQP0 ^ΔC/ΔC mouse lenses displayed loss of transparency. Lens fiber cell membrane vesicles (FCMVs) were prepared from wild type (WT), AQP0 heterozygous (AQP0 ^{+/ -} ), AQP0 knockout (AQP0 ^-/- ), AQP0 ^+/ΔC and AQP0 ^ΔC/ΔC ; water permeability (P f ) was measured using the osmotic shrinking method. CTCA assay was performed using adhesion-deficient L-cells and FCMVs prepared from the abovementioned genotypes. FCMVs of AQP0 ^{+/ -} and AQP0 ^-/- showed a statistically significant reduction (P < 0.001) in P f and CTCA compared to those of WT. AQP0 ^+/ΔC and AQP0 ^ΔC/ΔC FCMVs exhibited no statistically significant alteration (P > 0.05) in P f compared to those of WT. However, CTCA of AQP0 ^+/ΔC AQP0 ^ΔC/ΔC FCMVs was significantly higher (P < 0.001) than that of WT FCMVs. Our experiments clearly show that C-terminally end-cleaved AQP0 can function both as a water channel and a CTCA molecule in the lens fiber cell membranes. Also, end-truncation plays an important role in increasing the CTCA between fiber cells.
(Copyright © 2019 Elsevier Inc. All rights reserved.)

Exp Eye Res. 2010 Sep;91(3):393-404. (PMID: 20599966)
Genomics. 2003 Apr;81(4):361-8. (PMID: 12676560)
Biochem Biophys Res Commun. 2018 Nov 17;506(1):284-289. (PMID: 30348525)
Invest Ophthalmol Vis Sci. 2017 Dec 1;58(14):6006-6019. (PMID: 29196765)
Invest Ophthalmol Vis Sci. 2003 Nov;44(11):4820-8. (PMID: 14578404)
J Ultrastruct Res. 1984 Mar;86(3):228-45. (PMID: 6544861)
Invest Ophthalmol Vis Sci. 2000 Jan;41(1):175-82. (PMID: 10634618)
Mol Vis. 2007 Sep 11;13:1651-6. (PMID: 17893667)
Exp Eye Res. 2013 May;110:136-41. (PMID: 23116563)
Biochim Biophys Acta. 2011 Sep;1812(9):1089-97. (PMID: 21511033)
J Proteome Res. 2008 Jul;7(7):2696-702. (PMID: 18489132)
Invest Ophthalmol Vis Sci. 2004 Mar;45(3):884-91. (PMID: 14985306)
Curr Eye Res. 1985 Nov;4(11):1183-201. (PMID: 4075818)
Biochim Biophys Acta. 2014 Sep;1840(9):2862-77. (PMID: 24821012)
Invest Ophthalmol Vis Sci. 2014 Mar 03;55(3):1202-12. (PMID: 24458158)
Exp Eye Res. 1996 Dec;63(6):661-72. (PMID: 9068373)
Physiol Genomics. 2001 Dec 21;7(2):179-86. (PMID: 11773604)
Exp Eye Res. 2002 Nov;75(5):505-19. (PMID: 12457863)
Biochem Biophys Res Commun. 1987 Feb 13;142(3):761-6. (PMID: 3827901)
Exp Eye Res. 2013 Nov;116:371-85. (PMID: 24120416)
Biochem Biophys Res Commun. 2009 Dec 18;390(3):1034-9. (PMID: 19857466)
Invest Ophthalmol Vis Sci. 2010 Mar;51(3):1565-74. (PMID: 19875662)
Invest Ophthalmol Vis Sci. 2019 Mar 1;60(4):858-867. (PMID: 30821811)
Hum Mol Genet. 2000 Sep 22;9(15):2329-34. (PMID: 11001937)
Invest Ophthalmol Vis Sci. 1989 May;30(5):975-89. (PMID: 2722452)
Exp Eye Res. 1995 Sep;61(3):293-301. (PMID: 7556493)
Physiol Rev. 1997 Jan;77(1):21-50. (PMID: 9016299)
J Membr Biol. 2002 Mar 15;186(2):101-12. (PMID: 11944087)
Invest Ophthalmol Vis Sci. 2005 Apr;46(4):1393-402. (PMID: 15790907)
Biochem Biophys Res Commun. 2013 Nov 15;441(2):333-8. (PMID: 24148248)
Biochemistry. 1994 Jun 21;33(24):7663-9. (PMID: 8011633)
J Cell Biol. 1982 Apr;93(1):175-89. (PMID: 7068755)
J Membr Biol. 1999 Aug 1;170(3):191-203. (PMID: 10441663)
Biochemistry. 2004 Aug 3;43(30):9856-65. (PMID: 15274640)
Biochem Biophys Res Commun. 2014 Oct 3;452(4):986-91. (PMID: 25229686)
Pflugers Arch. 2009 Apr;457(6):1265-74. (PMID: 19034495)
Curr Eye Res. 1982-1983;2(12):843-7. (PMID: 7187641)
Invest Ophthalmol Vis Sci. 2006 Nov;47(11):4931-4. (PMID: 17065509)
Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14045-50. (PMID: 15377788)
Nature. 2004 May 13;429(6988):193-7. (PMID: 15141214)
Exp Eye Res. 2009 May;88(5):966-73. (PMID: 19135052)
Curr Eye Res. 1988 Dec;7(12):1255-62. (PMID: 3229136)
Biochem Biophys Res Commun. 2015 Jul 10;462(4):339-45. (PMID: 25960294)
Eur J Cell Biol. 1998 Aug;76(4):246-50. (PMID: 9765054)
J Mol Biol. 2007 Nov 16;374(1):162-9. (PMID: 17920625)

R01 EY026155 United States EY NEI NIH HHS

Keywords: AQP0; C-terminally end-cleaved AQP0; Cell-to-cell adhesion; Water permeability

0 (Aquaporins)
0 (Eye Proteins)
0 (aquaporin 0)
059QF0KO0R (Water)

Date Created: 20190304 Date Completed: 20200121 Latest Revision: 20200409

20240829

PMC6588171

10.1016/j.bbrc.2019.02.098

30826060