High Prevalence of Biofilm-Forming MRSA in the Conjunctival Flora in Chronic Dacryocystitis.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 8610759 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-5205 (Electronic) Linking ISSN: 08820538 NLM ISO Abbreviation: Semin Ophthalmol Subsets: MEDLINE

Publication: London : Informa Healthcare
Original Publication: [Orlando, FL] : Grune & Stratton, [c1986-

Biofilms*; Conjunctiva/*microbiology ; DNA, Bacterial/*genetics ; Dacryocystitis/*epidemiology ; Eye Infections, Bacterial/*epidemiology ; Methicillin-Resistant Staphylococcus aureus/*genetics ; Staphylococcal Infections/*epidemiology; Anti-Bacterial Agents/therapeutic use ; Case-Control Studies ; Chronic Disease ; Dacryocystitis/drug therapy ; Dacryocystitis/microbiology ; Eye Infections, Bacterial/microbiology ; Follow-Up Studies ; Humans ; India/epidemiology ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Microbial Sensitivity Tests ; Polymerase Chain Reaction ; Prevalence ; Prospective Studies ; Staphylococcal Infections/drug therapy ; Staphylococcal Infections/microbiology

Objectives: To report the microbiological spectrum of conjunctival flora and prevalence of biofilm-forming Methicillin-resistant Staphylococcus aureus (MRSA) in conjunctival flora in chronic dacryocystitis.
Design: Prospective, case-control study.
Methods: We included patients with unilateral chronic dacryocystitis, and their unaffected eyes as control. Microbiological profile and antibiotic susceptibility of the isolates was determined by standard microbiological procedures. S. aureus isolates were further evaluated for Methicillin resistance by Oxacillin resistance screening agar method and mecA polymerase chain reaction (PCR) and for biofilm synthesis by Congo red agar method, Microtitre plate (MTP) assay, and ica A and ica D PCR.
Results: We found 95 patients with unilateral chronic dacryocystitis. Aerobic Gram-positive isolates (74.2%, n = 72) were more than Gram-negative (25.7%, n = 25) or anaerobic isolates (20.5%, n = 25). S. aureus was most common (46.4%, n = 45), followed by Pseudomonas aeruginosa (10.3%, n = 10). Gram-positive isolates showed highest sensitivity to Linezolid (100%) and higher generation fluoroquinolones. Gram-negative isolates showed good sensitivity (>90%) to all tested antibiotics. S. aureus isolates showed MRSA prevalence as 93.5% and 96.9% by Oxacillin resistance screening agar method and mecA PCR, respectively. Biofilm formation was found in 71.8% MRSA isolates by MTP assay and 58.1% MRSA isolates were resistant to ≥3 classes of antibiotics.
Conclusions: Gram-positive organisms, specifically S. aureus, are the major etiological agent in chronic dacryocystitis. There is high prevalence of MRSA in these isolates and concurrent biofilm formation.

Keywords: MRSA; antibiotic susceptibility; biofilm formation; chronic dacryocystitis

0 (Anti-Bacterial Agents)
0 (DNA, Bacterial)

Date Created: 20190223 Date Completed: 20190403 Latest Revision: 20190403

20221213

10.1080/08820538.2019.1578382

30794029