Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Chromatography, Thin Layer*; Anti-Infective Agents/*chemistry ; Anti-Infective Agents/*pharmacology ; Curcuma/*chemistry ; Plant Extracts/*chemistry ; Plant Extracts/*pharmacology; Bacteria/drug effects ; Chromatography, Liquid ; Curcuma/metabolism ; Mass Spectrometry ; Methanol/chemistry ; Microbial Sensitivity Tests ; Solvents/chemistry
    • Abstract:
      Curcuma longa from Zingiberaceae belongs to the major spices consumed around the world, known from its cholagogue, anti-inflammatory, and antimicrobial properties. Lack of data on the activity of single components of turmeric extract encouraged the authors to apply TLC (thin-layer chromatography) based bioautography studies to reveal its antimicrobial constituents and construct a universal platform for the bioactivity assessment of crude extracts, with help of a freeware ImageJ software. This optimized chromatographic bioassay performed on diethyl ether and methanol extracts of Curcuma longa was successfully applied on the total extract and revealed the antimicrobial potential of single components against a variety of Gram-positive strains, with no need for their isolation from the mixture. The obtained results were further confronted with a classic microdilution antimicrobial assay on the isolates, purified from the crude extracts by centrifugal partition chromatography in the following solvent system: heptane-chloroform-methanol-water (5:6:3:2) ( v / v / v / v ).
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    • Grant Information:
      2015/17/D/NZ7/00822 Narodowe Centrum Nauki
    • Contributed Indexing:
      Keywords: Curcuma longa; ImageJ; TLC bioautography; TLC-MS; Zingiberaceae; antimicrobial agents; centrifugal partition chromatography; hydrostatic counter-current chromatography; turmeric tuber
    • Accession Number:
      0 (Anti-Infective Agents)
      0 (Plant Extracts)
      0 (Solvents)
      856YO1Z64F (turmeric extract)
      Y4S76JWI15 (Methanol)
    • Publication Date:
      Date Created: 20190223 Date Completed: 20190604 Latest Revision: 20200225
    • Publication Date:
      20240829
    • Accession Number:
      PMC6412823
    • Accession Number:
      10.3390/ijms20040898
    • Accession Number:
      30791406