Chemical shift assignments of a calmodulin intermediate with two Ca 2+ bound in complex with the IQ-motif of voltage-gated Ca 2+ channels (Ca V 1.2).

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  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: Netherlands NLM ID: 101472371 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1874-270X (Electronic) Linking ISSN: 1874270X NLM ISO Abbreviation: Biomol NMR Assign
    • Publication Information:
      Original Publication: Dordrecht : Springer
    • Subject Terms:
      Nuclear Magnetic Resonance, Biomolecular*; Calcium/*metabolism ; Calcium Channels/*chemistry ; Calmodulin/*chemistry; Amides/chemistry ; Amino Acid Motifs ; Humans ; Protein Binding ; Protein Structure, Secondary
    • Abstract:
      Calcium-dependent inactivation (CDI) of neuronal voltage-gated Ca 2+ channels (Ca V 1.2) is important for synaptic plasticity, which is associated with learning and memory. The Ca 2+ -dependent binding of calmodulin (CaM) to Ca V 1.2 is essential for CDI. Here we report NMR assignments for a CaM mutant (D21A/D23A/D25A/E32Q/D57A/D59A/N61A/E68Q, called CaM EF12 ) that contains two Ca 2+ bound at the third and fourth EF-hands (EF3 and EF4) and is bound to the IQ-motif (residues 1644-1665) from Ca V 1.2 (BMRB accession no. 27692).
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    • Grant Information:
      R01 AG055357 United States AG NIA NIH HHS; R01 EY012347 United States EY NEI NIH HHS; RF1 AG055357 United States AG NIA NIH HHS
    • Contributed Indexing:
      Keywords: CDI; CaV1.2; Calmodulin; EF-hand; IQ-motif; Synaptic plasticity
    • Accession Number:
      0 (Amides)
      0 (Calcium Channels)
      0 (Calmodulin)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20190222 Date Completed: 20190819 Latest Revision: 20240607
    • Publication Date:
      20240607
    • Accession Number:
      PMC6440834
    • Accession Number:
      10.1007/s12104-019-09883-0
    • Accession Number:
      30788773