Combination of Mesenchymal Stromal Cells and Cardiac Stem Cells in a Multilayer Cell Construct Promotes Activation of Notch Signaling and Initiation of Endothelial Differentiation.

Publisher: Springer Country of Publication: United States NLM ID: 0372557 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-8221 (Electronic) Linking ISSN: 00074888 NLM ISO Abbreviation: Bull Exp Biol Med Subsets: MEDLINE

Publication: New York : Springer
Original Publication: New York, Consultants Bureau.

Mesenchymal Stem Cells/*cytology ; Mesenchymal Stem Cells/*metabolism ; Myocytes, Cardiac/*cytology; Animals ; Basic Helix-Loop-Helix Transcription Factors/metabolism ; Cell Differentiation/physiology ; Cell Proliferation/genetics ; Cell Proliferation/physiology ; Cells, Cultured ; Myocardial Infarction/metabolism ; Myocytes, Cardiac/metabolism ; Rats ; Repressor Proteins/metabolism ; Signal Transduction/physiology ; Tissue Engineering ; Transcription Factor HES-1/metabolism

We showed the possibility of generating combined tissue-engineered cell consisting of layers of rat cardiac stem cells and mesenchymal stromal cells from the adipose tissue. Cell-cell interaction within the cell sheet promoted proliferation of cardiac stem cells, expression of endothelial differentiation marker ETS1, and Notch signaling activation. The obtained results provide new insights into possible mechanisms of stimulation of endogenous regeneration processes after myocardial damage and demonstrate potential of cell-based cardiomyoplasty with the use of these combined cell sheets.

Keywords: Notch signaling; cardiac stem cells; cell sheets; mesenchymal stromal cells; myocardial infarction

0 (Basic Helix-Loop-Helix Transcription Factors)
0 (Hairy, HRT1 protein)
0 (Hes1 protein, rat)
0 (Repressor Proteins)
0 (Transcription Factor HES-1)

Date Created: 20190221 Date Completed: 20190715 Latest Revision: 20190715

20231215

10.1007/s10517-019-04390-7

30783844