A novel template repairing-PCR (TR-PCR) reaction platform for microRNA detection using translesional synthesis on DNA templates containing abasic sites.

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  • Additional Information
    • Source:
      Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 9610838 Publication Model: Print Cited Medium: Internet ISSN: 1364-548X (Electronic) Linking ISSN: 13597345 NLM ISO Abbreviation: Chem Commun (Camb) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Cambridge : Royal Society of Chemistry
    • Subject Terms:
      DNA Replication* ; Templates, Genetic*; DNA/*chemistry ; MicroRNAs/*analysis ; Reverse Transcriptase Polymerase Chain Reaction/*methods
    • Abstract:
      A novel template repairing-PCR technology based on miRNA-primed bypass synthesis at the abasic sites on the PCR template is developed as a sensitive and selective platform for miRNA detection. The assay is expected to show great promise for reverse transcription-free RNA PCR amplification and target-based coding analysis.
    • Accession Number:
      0 (MicroRNAs)
      9007-49-2 (DNA)
    • Publication Date:
      Date Created: 20190219 Date Completed: 20190405 Latest Revision: 20190405
    • Publication Date:
      20221213
    • Accession Number:
      10.1039/c8cc10226k
    • Accession Number:
      30775737