Effects of N-acetyl-cysteine supplementation on sperm quality, chromatin integrity and level of oxidative stress in infertile men.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101153627 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-7827 (Electronic) Linking ISSN: 14777827 NLM ISO Abbreviation: Reprod Biol Endocrinol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, 2003-
    • Subject Terms:
      Dietary Supplements*; Acetylcysteine/*administration & dosage ; Chromatin/*drug effects ; Infertility, Male/*drug therapy ; Oxidative Stress/*drug effects ; Spermatozoa/*drug effects; Adult ; Chromatin/genetics ; Chromatin/metabolism ; DNA Damage ; Free Radical Scavengers/administration & dosage ; Humans ; Infertility, Male/genetics ; Infertility, Male/physiopathology ; Iran ; Male ; Reactive Oxygen Species/antagonists & inhibitors ; Reactive Oxygen Species/metabolism ; Semen Analysis ; Sperm Motility/drug effects ; Spermatozoa/metabolism ; Spermatozoa/physiology
    • Abstract:
      Background: Infertile men have higher levels of semen reactive oxygen species (ROS) than fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This study investigated the effects of supplementation with N-acetyl-cysteine (NAC) on the sperm quality, chromatin integrity and levels of oxidative stress in infertile men.
      Methods: The study was carried out in the unit of ACECR Infertility Research Center, Qom, Iran. The patients consisted of 50 infertile men with asthenoteratozoospermia who received NAC (600 mg/d) orally for 3 months, after which they were compared with pre-treatment status. Semen was analyzed according to WHO (2010), followed by the assessment of protamine content [chromomycin A3 (CMA3)] and DNA integrity [terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)]. Oxidative stress markers, i.e. total antioxidant capacity (TAC) and malondialdehyde (MDA), as well as hormonal profile (LH, FSH, Testosterone and Prolactin) were determined by ELISA kit.
      Results: After NAC treatment, patients' sperm count and motility increased significantly whereas abnormal morphology, DNA fragmentation and protamine deficiency showed significant decreases compared to pre-treatment levels (P < 0.05). Hormonal profile improvement was associated with lowered FSH and LH levels and increased amount of testosterone (P < 0.05). TAC significantly increased and MDA decreased with an inverse significant correlation between TAC and MDA (P < 0.05).
      Conclusion: NAC oral supplementation may improve sperm parameters and oxidative/antioxidant status in infertile males.
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    • Contributed Indexing:
      Keywords: Asthenoteratozoospermia; Infertility; N-acetyl-cysteine; Oxidative stress
    • Accession Number:
      0 (Chromatin)
      0 (Free Radical Scavengers)
      0 (Reactive Oxygen Species)
      WYQ7N0BPYC (Acetylcysteine)
    • Publication Date:
      Date Created: 20190218 Date Completed: 20190401 Latest Revision: 20200225
    • Publication Date:
      20231215
    • Accession Number:
      PMC6377938
    • Accession Number:
      10.1186/s12958-019-0468-9
    • Accession Number:
      30771790