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A bisubstrate reagent orchestrating adenosine triphosphate and l-tyrosine and making tyrosyl adenylate: partial mimicking of tyrosyl-tRNA synthetase.
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- Author(s): Singh H;Singh H; Kaur B; Kaur H; Singh P
- Source:
Organic & biomolecular chemistry [Org Biomol Chem] 2018 Dec 12; Vol. 16 (48), pp. 9446-9453.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101154995 Publication Model: Print Cited Medium: Internet ISSN: 1477-0539 (Electronic) Linking ISSN: 14770520 NLM ISO Abbreviation: Org Biomol Chem Subsets: MEDLINE
- Publication Information:
Original Publication: Cambridge, UK : Royal Society of Chemistry, c2003-
- Subject Terms:
- Abstract:
We report the development of a bisubstrate reagent that, similar to tyrosyl t-RNA synthetase (TyrTS), provides a surface for ATP and l-Tyr to render a pseudo-intramolecular reaction forming 5'-tyrosyl adenylate (tyrAd). The presence of the reagent in solution with TyrTS marred the enzymatic reaction and, noticeably, tyrAd formed under the catalytic mode of the biomodel reagent was not picked up by TyrTS and hence was not transferred to tRNA. A potential application of this reagent, which doesn't allow the formation of tyrosyl tRNA, may lie in an emerging therapeutic targeting the translation machinery of cells without inhibiting the normal workings of enzymes.
- Accession Number:
0 (Indicators and Reagents)
415SHH325A (Adenosine Monophosphate)
42HK56048U (Tyrosine)
6372-08-3 (tyrosinyl-5'-AMP)
8L70Q75FXE (Adenosine Triphosphate)
EC 6.1.1.1 (Tyrosine-tRNA Ligase)
- Publication Date:
Date Created: 20181206 Date Completed: 20190313 Latest Revision: 20190313
- Publication Date:
20221213
- Accession Number:
10.1039/c8ob02866d
- Accession Number:
30515504
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