Immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide for selective extraction of phosphoproteins.

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  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7808782 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-5073 (Electronic) Linking ISSN: 00263672 NLM ISO Abbreviation: Mikrochim Acta Subsets: MEDLINE
    • Publication Information:
      Original Publication: Wien ; New York : Springer-Verlag.
    • Subject Terms:
      Ethylenediamines/*chemistry ; Graphite/*chemistry ; Phosphoproteins/*isolation & purification ; Tungsten Compounds/*chemistry; Adsorption ; Animals ; Caseins/blood ; Caseins/isolation & purification ; Chromatography, Liquid ; Electrophoresis, Polyacrylamide Gel ; Humans ; Myocardium/chemistry ; Nanostructures/chemistry ; Nanostructures/ultrastructure ; Oxides/chemistry ; Phosphoproteins/blood ; Solid Phase Extraction ; Swine ; Tandem Mass Spectrometry
    • Abstract:
      A sorbent for selective extraction of phosphoproteins was obtained by immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO). The resulting composites exhibit an adsorption capacity of 981 mg g -1 for β-casein due to the synergistic effect of metal-affinity interaction between Ce(IV) and phosphate groups and π-stacking interaction between the polyoxometalate framework and the phosphate groups. The results of LC-MS and SDS-PAGE analysis show that the CeEGO composites can be applied to the extraction of phosphoproteins from protein mixture, and as little as 50 μg mL -1 of the phosphoprotein β-casein can be detected by SDS-PAGE. It was also applied to the extraction of β-casein from spiked biological samples such as drinking milk, whole blood and swine heart tissue extract. Graphical abstract An efficient sorbent is obtained by immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO). The resulting composites exhibit highly selective capture capacity towards phosphoproteins due to the synergistic effect of metal-affinity interaction between Ce(IV) and phosphate groups and π-stacking interaction between the polyoxometalate framework and the phosphate groups.
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    • Grant Information:
      21605014 International National Natural Science Foundation of China; 21475018 International National Natural Science Foundation of China; 21727811 International National Natural Science Foundation of China; N160504010 International Fundamental Research Funds for the Central Universities
    • Contributed Indexing:
      Keywords: Functionalized GO; LC-MS; Metal-affinity interaction; Phosphoproteins extraction; Polyoxymetalate framework; SDS-PAGE
    • Accession Number:
      0 (Caseins)
      0 (Ethylenediamines)
      0 (Oxides)
      0 (Phosphoproteins)
      0 (Tungsten Compounds)
      0 (polyoxometalate I)
      60V9STC53F (ethylenediamine)
      7782-42-5 (Graphite)
    • Publication Date:
      Date Created: 20181121 Date Completed: 20190204 Latest Revision: 20190215
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/s00604-018-3095-9
    • Accession Number:
      30456423