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[Screening of microRNAs targeting Notch signaling pathway implicated in inner ear development and the role of microRNA-384-5p].
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- Additional Information
- Source:
Publisher: Zhonghua yi xue hui za zhi she Country of Publication: China NLM ID: 101247574 Publication Model: Print Cited Medium: Print ISSN: 1673-0860 (Print) Linking ISSN: 16730860 NLM ISO Abbreviation: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi Subsets: MEDLINE
- Publication Information:
Original Publication: Beijing : Zhonghua yi xue hui za zhi she, 2005-
- Subject Terms:
- Abstract:
Objective: To screen the key microRNAs targeting Notch signaling pathway in inner ear and investigate its potential regulating function. Methods: The interaction network and the Core-Notch network, involved with key genes in Notch signal pathway and differential-expressed microRNAs in inner ear, were constructed by bioinformatics methods. The important microRNAs in regulating Notch signaling pathway were screened via topological and GO analysis, followed by in vivo and in vitro investigation. Results: MiRNA-384-5p was identified as a key regulator specifically expressed in mouse brain and inner ear, which could down-regulate Notch1 . The Notch1 expression was found significantly down-regulated in miRNA-384-5p-mimic-transfected HeLa cells. The dual-luciferase reporter gene assay further confirmed the effect of miRNA-384-5p on the down-regulation of Notch1 and Dll4 in Notch signaling pathway. Conclusions: The Core-Notch network is constructed to screen microRNAs implicated in inner ear development, and miRNA-384-5p is screened and verified to be target-regulating the Notch signaling pathway, which could be the potential target in the regeneration of impaired hair cells.
- Grant Information:
BE2016762 Key Research and Development Program of Jiangsu Province (Social Development)
- Contributed Indexing:
Keywords: Ear, inner; Hair cells, auditory; Receptors, Notch; miR-384-5p
Local Abstract: [Publisher, Chinese] 目的: 筛选内耳中靶向调控Notch信号通路的关键microRNA,验证其对Notch信号通路的调控作用。 方法: 构建Notch信号通路相关基因与microRNA相互作用网络,筛选核心基因并提取Core-Notch子网络,再通过拓扑学分析与GO分析筛选对Notch信号通路具有调控作用的关键microRNA,并在体内、外水平进行验证。 结果: 以筛选的microRNA-384-5p(miR-384-5p)为研究对象,预测其对Notch信号通路具有较强调控作用。实验结果显示,miR-384-5p在小鼠脑与耳蜗组织中特异性表达;miR-384-5p-mimic转染HeLa细胞后, Notch1 的表达水平显著下调;双荧光素酶报告基因检测进一步验证了miR-384-5p对Notch信号通路中 Notch1 和 Dll4 的负调控作用。 结论: 基于构建的Core-Notch网络筛选内耳发育相关的microRNA,筛选出来的miR-384-5p分子可靶向调控Notch信号通路,可作为潜在的毛细胞修复的干预靶点。.
- Accession Number:
0 (Adaptor Proteins, Signal Transducing)
0 (Calcium-Binding Proteins)
0 (DLL4 protein, human)
0 (DLL4 protein, mouse)
0 (Intercellular Signaling Peptides and Proteins)
0 (Intracellular Signaling Peptides and Proteins)
0 (MIRN384 microRNA, human)
0 (Membrane Proteins)
0 (MicroRNAs)
0 (NOTCH1 protein, human)
0 (Notch1 protein, mouse)
0 (Receptor, Notch1)
EC 1.13.12.- (Luciferases)
- Publication Date:
Date Created: 20181120 Date Completed: 20190226 Latest Revision: 20201209
- Publication Date:
20221213
- Accession Number:
10.3760/cma.j.issn.1673-0860.2018.11.007
- Accession Number:
30453402
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