Subcellular fractionation of the longitudinal smooth muscle/myenteric plexus of dog ileum: dissociation of the distribution of two plasma membrane marker enzymes.

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  • Additional Information
    • Source:
      Publisher: Wiley on behalf of the International Society for Neurochemistry Country of Publication: England NLM ID: 2985190R Publication Model: Print Cited Medium: Print ISSN: 0022-3042 (Print) Linking ISSN: 00223042 NLM ISO Abbreviation: J Neurochem Subsets: MEDLINE
    • Publication Information:
      Publication: 2001- : Oxford, UK : Wiley on behalf of the International Society for Neurochemistry
      Original Publication: New York : Raven Press
    • Subject Terms:
      Cell Fractionation*; Cell Membrane/*enzymology ; Ileum/*ultrastructure ; Muscle, Smooth/*ultrastructure ; Myenteric Plexus/*ultrastructure; 4-Nitrophenylphosphatase/analysis ; Animals ; Cell Membrane/ultrastructure ; Centrifugation, Density Gradient ; Dogs ; Female ; Ileum/enzymology ; Male ; Microscopy, Electron ; Microsomes/ultrastructure ; Nucleotidases/analysis ; Saxitoxin/metabolism ; Synaptosomes/enzymology ; Synaptosomes/ultrastructure
    • Abstract:
      The distribution of plasma membrane markers, the sodium pump [evaluated as ouabain-sensitive, potassium-stimulated p-nitrophenyl phosphatase (K+-pNPPase)], [3H]saxitoxin binding, and 5'-AMPase, was studied in the subcellular fractions prepared from the homogenates of the longitudinal smooth muscle/myenteric plexus of dog ileum. The K+-pNPPase activity and [3H]-saxitoxin binding were found to be predominantly associated with the synaptosomal fraction as indicated by the high level of these activities in the crude synaptosomal fraction and by the copurification of K+-pNPPase and [3H]saxitoxin binding, but not 5'-AMPase, with several synaptosomal markers during the fractionation of the crude synaptosomal fraction on density gradients. In contrast to the K+-pNPPase activity and [3H]saxitoxin binding, the 5'-AMPase activity was found to be concentrated in the microsomal pellet. Further fractionation of microsomes on density gradient resulted in copurification of 5'-AMPase but not K+-pNPPase or [3H]saxitoxin binding, with other smooth muscle plasma membrane-bound enzymes, such as high-affinity Ca2+-ATPase, Mg2+-ATPase, and Ca2+-ATPase. It was concluded that in the longitudinal smooth muscle/myenteric plexus, the sodium pump activity is present in higher density in the neuronal plasma membranes whereas 5'-AMPase activity is concentrated in the smooth muscle plasma membranes.
    • Accession Number:
      35523-89-8 (Saxitoxin)
      EC 3.1.3.- (Nucleotidases)
      EC 3.1.3.- (adenosine monophosphatase)
      EC 3.1.3.41 (4-Nitrophenylphosphatase)
    • Publication Date:
      Date Created: 19871001 Date Completed: 19871019 Latest Revision: 20190630
    • Publication Date:
      20231215
    • Accession Number:
      10.1111/j.1471-4159.1987.tb10002.x
    • Accession Number:
      3040906