Extracellular vesicle secretion of miR-142-3p from lung adenocarcinoma cells induces tumor promoting changes in the stroma through cell-cell communication.

Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8811105 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-2744 (Electronic) Linking ISSN: 08991987 NLM ISO Abbreviation: Mol Carcinog Subsets: MEDLINE

Publication: <2005- > : [Hoboken, N.J.] : Wiley-Liss
Original Publication: New York : Alan R. Liss, Inc., c1988-

Cell Communication*; Adenocarcinoma/*pathology ; Cancer-Associated Fibroblasts/*pathology ; Extracellular Vesicles/*metabolism ; Lung Neoplasms/*pathology ; MicroRNAs/*genetics ; Stromal Cells/*pathology; Adenocarcinoma/genetics ; Adenocarcinoma/metabolism ; Apoptosis ; Cancer-Associated Fibroblasts/metabolism ; Carcinogens/metabolism ; Cell Movement ; Cell Proliferation ; Cell Transformation, Neoplastic ; Gene Expression Regulation, Neoplastic ; Humans ; Lung Neoplasms/genetics ; Lung Neoplasms/metabolism ; MicroRNAs/metabolism ; Stromal Cells/metabolism ; Tumor Cells, Cultured ; Tumor Microenvironment ; Wound Healing

Extracellular vesicles (EVs) are mediators of communication between cancer cells and the surrounding tumor microenvironment. EV content is able to influence key tumorigenic changes including invasion, metastasis, and inducing pro-tumor changes in the stroma. MiR-142-3p is a known tumor suppressor in LAC and was recently shown to be enriched within LAC EVs, indicating its potential as a key signaling miRNA. Our research demonstrates the role EV associated miR-142-3p plays when transferred from LAC cells to both endothelial and fibroblast cells. We demonstrate that transfer of miR-142-3p in LAC EVs to endothelial cells promotes angiogenesis through inhibition of TGFβR1. Additionally, we show EV associated miR-142-3p promotes the cancer-associated fibroblast phenotype in lung fibroblast cells which we show is independent of TGFβ signaling. These findings suggest that miR-142-3p within LAC EVs can be transferred from LAC cells to both endothelial and fibroblast cells to promote tumor associated changes.
(© 2018 Wiley Periodicals, Inc.)

1002 International Terry Fox Research Institute (Early Detection of Lung Cancer-A Pan-Canadian Study)

Keywords: MiRNA; NSCLC; exosomes; extracellular vesicles; lung adenocarcinoma

0 (Carcinogens)
0 (MIRN142 microRNA, human)
0 (MicroRNAs)

Date Created: 20181027 Date Completed: 20190708 Latest Revision: 20190708

20231215

10.1002/mc.22935

30362621