Two-photon excitation and direct emission from S 2 state of U.S. Food and Drug Administration approved near-infrared dye: Application of anti-Kasha's rule for two-photon fluorescence imaging.

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  • Author(s): Kumari A;Kumari A; Gupta S; Gupta S; Gupta S
  • Source:
    Journal of biophotonics [J Biophotonics] 2019 Jan; Vol. 12 (1), pp. e201800086. Date of Electronic Publication: 2018 Sep 18.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101318567 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1864-0648 (Electronic) Linking ISSN: 1864063X NLM ISO Abbreviation: J Biophotonics Subsets: MEDLINE
    • Publication Information:
      Original Publication: Weinheim : Wiley-VCH
    • Subject Terms:
      Drug Approval* ; Infrared Rays* ; Photons* ; United States Food and Drug Administration*; Coloring Agents/*chemistry ; Indocyanine Green/*chemistry ; Microscopy, Fluorescence, Multiphoton/*methods; HeLa Cells ; Humans ; Optical Phenomena ; United States
    • Abstract:
      In recent years, two-photon fluorescence microscopy has gained significant interest in bioimaging. It allows the visualization of deeply buried inhomogeneities in tissues. The near-infrared (NIR) dyes are also used for deep tissue imaging. Indocyanine green (ICG) is the only U.S. Food and Drug Administration (FDA) approved exogenous contrast agent in the NIR region for clinical applications. However, despite its potential candidature, it had never been used as a two-photon contrast agent for biomedical imaging applications. This letter provides an insight into the scope and application of the two-photon excitation property of ICG to the second excited singlet (S 2 ) state in aqueous solution. Furthermore, in this work, we demonstrate the two-photon cellular imaging application of ICG using direct fluorescence emission from S 2 state for the first time. Our results show that two-photon excitation to S 2 state of ICG could be achieved with approximately 790 nm wavelength of femtosecond laser, which lies in well-known "tissue-optical window." This property would enable light to penetrate much deeper in the turbid medium such as biological tissues. Thus, ICG could be used as the first FDA approved NIR exogenous contrast agent for two-photon imaging. These findings can make remarkable influence on preclinical and clinical cell imaging.
      (© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
    • Contributed Indexing:
      Keywords: anti-Kasha's rule; dual emission; indocyanine green; non-linear optics; two-photon fluorescence microscopy
    • Accession Number:
      0 (Coloring Agents)
      IX6J1063HV (Indocyanine Green)
    • Publication Date:
      Date Created: 20180830 Date Completed: 20200325 Latest Revision: 20200325
    • Publication Date:
      20221213
    • Accession Number:
      10.1002/jbio.201800086
    • Accession Number:
      30155994