Ectopic activation of cortical cell division during the accommodation of arbuscular mycorrhizal fungi.

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  • Additional Information
    • Source:
      Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford : Wiley on behalf of New Phytologist Trust
      Original Publication: London, New York [etc.] Academic Press.
    • Subject Terms:
      Signal Transduction* ; Symbiosis*; Medicago truncatula/*microbiology ; Mycorrhizae/*physiology ; Plant Proteins/*metabolism; Cell Division ; Gene Expression Regulation, Plant ; Medicago truncatula/genetics ; Plant Proteins/genetics ; Plant Roots/metabolism
    • Abstract:
      Arbuscular mycorrhizas (AMs) between plants and soil fungi are widespread symbioses with a major role in soil nutrient uptake. In this study we investigated the induction of root cortical cell division during AM colonization by combining morphometric and gene expression analyses with promoter activation and protein localization studies of the cell-plate-associated exocytic marker TPLATE. Our results show that TPLATE promoter is activated in colonized cells of the root cortex where we also observed the appearance of cells that are half the size of the surrounding cells. Furthermore, TPLATE-green fluorescent protein recruitment to developing cell plates highlighted ectopic cell division events in the inner root cortex during early AM colonization. Lastly, transcripts of TPLATE, KNOLLE and Cyclinlike 1 (CYC1) are all upregulated in the same context, alongside endocytic markers Adaptor-Related Protein complex 2 alpha 1 subunit (AP2A1) and Clathrin Heavy Chain 2 (CHC2), known to be active during cell plate formation. This pattern of gene expression was recorded in wild-type Medicago truncatula roots, but not in a common symbiotic signalling pathway mutant where fungal colonization is blocked at the epidermal level. Altogether, these results suggest the activation of cell-division-related mechanisms by AM hosts during the accommodation of the symbiotic fungus.
      (© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.)
    • Grant Information:
      International Compagnia di Sanpaolo (project REPROGRAM - Progetti di Ateneo 2012, Call 01); 2013-2014-2017 International UNITO
    • Contributed Indexing:
      Keywords: Daucus carota; Lotus japonicus; Medicago truncatula; arbuscular mycorrhiza; cell division; endocytosis; live confocal imaging; symbiosis
    • Accession Number:
      0 (Plant Proteins)
    • Publication Date:
      Date Created: 20180829 Date Completed: 20200218 Latest Revision: 20200930
    • Publication Date:
      20231215
    • Accession Number:
      10.1111/nph.15398
    • Accession Number:
      30152051