Zinc Oxide Tetrapods Based Biohybrid Interface for Voltammetric Sensing of Helicobacter pylori.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, D.C. : American Chemical Society
    • Subject Terms:
      Electrochemical Techniques*; Biosensing Techniques/*methods ; Helicobacter pylori/*physiology ; Zinc Oxide/*chemistry; Electrodes ; Helicobacter pylori/chemistry ; Helicobacter pylori/isolation & purification ; Humans ; Limit of Detection
    • Abstract:
      Helicobacter pylori is a Gram-negative, spiral shaped, microaerophilic bacteria that colonizes human gastric mucosa and causes various gastric diseases. In this work, the utilization of ion irradiated zinc oxide tetrapods (ZnO-T) based biohybrid interface accentuates the development of an electrochemical immunosensor for the fast and sensitive detection of H. pylori. After coating of (ZnO-T) over the surface of screen printed electrode (SP-AuE) through electrodeposition, the ZnO-T/SP-AuE was irradiated with N 2 + ion of energy 100 keV. The ion irradiation significantly enhances the conductivity of ZnO-T coated SP-AuE. The revamped SP-AuE is further used for establishing an immunosensor interface based upon immobilization of the CagA antigen on ZnO-T electrodeposited over the surface of SP-AuE. The sensing interface demonstrated good linearity (0.2 ng/mL to 50 ng/mL) and limit of detection (0.2 ng/mL). The ion beam irradiated ZnO-T based immunosensor showed significantly high conductivity and enhanced the analytical properties of the working electrode in terms of the sensitivity, detection limit, and response time. A study on the comparison of irradiated and pristine electrode is performed for amperometric sensing of H. pylori. In addition, the significance of work conducted on ion irradiated ZnO-T based interfaces provides a basis of further development of electrochemical immunosensors.
    • Contributed Indexing:
      Keywords: CagA antigen; H. pylori; immunosensor; ion beam modification; screen printed electrode; zinc oxide tetrapods
    • Accession Number:
      SOI2LOH54Z (Zinc Oxide)
    • Publication Date:
      Date Created: 20180822 Date Completed: 20190326 Latest Revision: 20190326
    • Publication Date:
      20240829
    • Accession Number:
      10.1021/acsami.8b08901
    • Accession Number:
      30130396