Development of RBC Membrane Antigen Arrays for Validating Blood Grouping Reagents.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res
    • Publication Information:
      Original Publication: Washington, D.C. : American Chemical Society, c2002-
    • Subject Terms:
      Antibodies/*chemistry ; Antigens/*immunology ; Blood Grouping and Crossmatching/*methods ; Erythrocyte Membrane/*immunology ; Protein Array Analysis/*methods; Amino Acid Sequence ; Antibodies/metabolism ; Antibody Specificity ; Antigens/chemistry ; Antigens/classification ; Blood Grouping and Crossmatching/instrumentation ; Cross Reactions ; Erythrocyte Membrane/chemistry ; Humans ; Protein Array Analysis/instrumentation ; Sensitivity and Specificity
    • Abstract:
      Antibody reagents have been remained as a standard approach to characterize blood group (BG) antigens in clinic. The specificity and cross-reactivity of these BG antibodies are routine detected using the gel microcolumn assay (GMA). However, the GMA is neither specific nor sensitive, thus increasing the risk of improperly matched RBC transfusions. In this work, we describe a bead-based RBC membrane antigen array to detect BG antibody-antigen binding with ∼700-fold higher sensitivity and dynamic range than the GMA. RBC membrane antigen arrays were fabricated using fragmented RBC membranes highly enriched in BG panel antigens. The arrays were then used to screen the interactions of 15 BG reagents to three antigen panels. The majority of the antibody reactions (i.e., 86.7%; 39/45) aligned with those obtained with the GMA. The six cross-reactive, nonspecific antibody reactions identified only by our arrays (i.e., 13.3%; 6/45) were confirmed by agglutination inhibition and genotyping assays. These results demonstrate that our RBC membrane antigen array has great potential in screening BG antibodies and improving the safety of RBC transfusions.
    • Contributed Indexing:
      Keywords: antibody; antigen array; blood group; proteomics; red blood cell
    • Accession Number:
      0 (Antibodies)
      0 (Antigens)
    • Publication Date:
      Date Created: 20180818 Date Completed: 20190801 Latest Revision: 20190801
    • Publication Date:
      20221213
    • Accession Number:
      10.1021/acs.jproteome.8b00370
    • Accession Number:
      30114910