Lyophilisation as a simple and safe method for long-term storage of free-living amoebae at ambient temperature.

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  • Additional Information
    • Source:
      Publisher: Springer International Country of Publication: Germany NLM ID: 8703571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1955 (Electronic) Linking ISSN: 09320113 NLM ISO Abbreviation: Parasitol Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin : Springer International, c1987-
    • Subject Terms:
      Acanthamoeba/*physiology ; Amoebozoa/*physiology ; Preservation, Biological/*methods; Acanthamoeba/chemistry ; Amoebozoa/chemistry ; Animals ; Temperature
    • Abstract:
      Free-living amoebae (FLA) are protozoa ubiquitously found in nature. As some species or strains of these FLA are pathogenic for humans and animals, they represent objects of medical and parasitological research worldwide. Storage of valuable FLA strains in laboratories is often time- and energy-consuming and expensive. The shipment of such strains as frozen stocks is cumbersome and challenging in terms of cooling requirements as well as of transport regulations. To overcome these difficulties and challenges in maintenance and transport, we present a new method to generate lyophilised samples of non-cyst-forming FLA (Ripella (Vannella) spp.) and cyst-forming FLA (Acanthamoeba spp.) strains which guarantees a simple mechanism for long-term storage at ambient temperature, as well as easy handling and/or shipment. The survival rate of all FLA lyophilisates after short-term storage (2 months) was comparable to the survival rate of freeze cultures of the respective strains. Furthermore, the viability of Acanthamoeba spp. cysts after storage for 29 months was 20 to 40% following lyophilisation and rehydration, with strain variation.
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    • Contributed Indexing:
      Keywords: Acanthamoeba; Free-living amoebae; Log-term storage; Lyophilisation; Ripella
    • Publication Date:
      Date Created: 20180811 Date Completed: 20181211 Latest Revision: 20191210
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s00436-018-6029-x
    • Accession Number:
      30094540