nNOS immunoreactivity co-localizes with GABAergic and cholinergic neurons, and associates with β-endorphinergic and met-enkephalinergic opioidergic fibers in rostral ventromedial medulla and A5 of the mouse.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 0045503 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6240 (Electronic) Linking ISSN: 00068993 NLM ISO Abbreviation: Brain Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam Elsevier/North-Holland Biomedical Press.
    • Subject Terms:
      Brain Stem/*metabolism ; Cholinergic Neurons/*metabolism ; GABAergic Neurons/*metabolism ; Midbrain Raphe Nuclei/*metabolism ; Nitric Oxide Synthase Type I/*metabolism ; beta-Endorphin/*metabolism; Animals ; Brain Stem/cytology ; Cholinergic Neurons/cytology ; Enkephalins/metabolism ; GABAergic Neurons/cytology ; Male ; Medulla Oblongata/metabolism ; Mice ; Midbrain Raphe Nuclei/cytology ; Pain/metabolism ; Prefrontal Cortex/metabolism ; Receptors, Opioid/metabolism ; Serotonergic Neurons/metabolism ; Serotonin/metabolism
    • Abstract:
      The present study examined the co-expression of neuronal nitric oxide synthase (nNOS) in the rostral ventromedial medulla (RVM) and A5 regions of the mouse brainstem within several neurochemical populations involved in nociceptive modulation. Double immunohistochemical methods showed that nNOS+ neurons do not co-localize with serotonergic neurons within any of these regions. Within the RVM, the nuclei raphe magnus and gigantocellularis contain a population of nNOS+/GAD67+ neurons, and within the paragigantocellularis lateralis, there is a smaller population of nNOS+/CHAT+ neurons. Further, nNOS+ neurons overlap the region of expression of β-endorphinergic and met-enkephalinergic fibers within the RVM. No co-labeling was found within the A5 for any of these populations. These findings suggest that pain-modulatory serotonergic neurons within the brainstem do not directly produce nitric oxide (NO). Rather, NO-producing neurons within the RVM belong to GABAergic and cholinergic cell populations, and are in a position to modulate or be modulated by local opioidergic neurons.
      (Copyright © 2018 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Nitric oxide; Pain; RVM
    • Accession Number:
      0 (Enkephalins)
      0 (Receptors, Opioid)
      333DO1RDJY (Serotonin)
      60617-12-1 (beta-Endorphin)
      EC 1.14.13.39 (Nitric Oxide Synthase Type I)
      EC 1.14.13.39 (Nos1 protein, mouse)
    • Publication Date:
      Date Created: 20180807 Date Completed: 20191104 Latest Revision: 20191104
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.brainres.2018.08.003
    • Accession Number:
      30081038