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Enzyme-linked immunosorbent assay and agar gel immunodiffusion assay for diagnosis of equine infectious anemia employing p26 protein fused to the maltose-binding protein.
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- Additional Information
- Source:
Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-8798 (Electronic) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE
- Publication Information:
Original Publication: Wien, New York, Springer-Verlag.
- Subject Terms:
- Abstract:
A codon-optimized equine infectious anemia virus p26 gene was fused to a maltose-binding protein (MBP) and expressed in Escherichia coli for use as an antigen in agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA) for diagnosis of equine infectious anemia. An analysis of analytical sensitivity and specificity showed that the antigen MBP-p26rec reacted positively with a reference World Organization for Animal Health serum and demonstrated no cross-reaction against sera from vaccinated animals in either test. The diagnostic characteristics were evaluated and presented excellent values. The AGIDrec showed 100% sensitivity and specificity, and the ELISArec showed 100% sensitivity and 99.64% specificity. In addition, MBP-p26rec was stabile after three years of storage at 4 °C, maintaining its immunoreactivity.
- Grant Information:
APS 0338-5.05/08 Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
- Accession Number:
0 (Maltose-Binding Proteins)
0 (Viral Core Proteins)
0 (equine infectious anemia virus, p26)
- Publication Date:
Date Created: 20180709 Date Completed: 20180913 Latest Revision: 20191210
- Publication Date:
20221213
- Accession Number:
10.1007/s00705-018-3923-6
- Accession Number:
29982961
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