Elevated hydrostatic pressure promotes ameloblastoma cell invasion through upregulation of MMP-2 and MMP-9 expression via Wnt/β-catenin signalling.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: Denmark NLM ID: 8911934 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0714 (Electronic) Linking ISSN: 09042512 NLM ISO Abbreviation: J Oral Pathol Med Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford, UK : Wiley-Blackwell
      Original Publication: Copenhagen : Munksgaard, c1989-
    • Subject Terms:
      Gene Expression* ; Hydrostatic Pressure* ; Up-Regulation*; Ameloblastoma/*genetics ; Ameloblastoma/*pathology ; Matrix Metalloproteinase 2/*genetics ; Matrix Metalloproteinase 2/*metabolism ; Matrix Metalloproteinase 9/*genetics ; Matrix Metalloproteinase 9/*metabolism ; Wnt Signaling Pathway/*physiology ; beta Catenin/*metabolism; Cell Movement ; Humans ; Neoplasm Invasiveness ; Tumor Cells, Cultured
    • Abstract:
      Background: The process of marsupialization involves the release of intracystic pressure and the fluid contained within. Marsupialization of cystic ameloblastoma is controversial; therefore, we investigated how hydrostatic pressure influences biological behaviours of ameloblastoma cells and its underlying mechanisms.
      Materials and Methods: An ameloblastoma epithelial cell line, hTERT + -AM, was exposed to different hydrostatic pressures with or without Dickkopf-related protein 1 (also known as DKK), a canonical Wnt signalling pathway inhibitor. A CCK-8 assay, a monolayer wound assay, and a Transwell assay were used to determine cell proliferation, migration and invasion, respectively. qRT-PCR and Western blot were used to detect expression of MMP-2, MMP-9, RANKL and other downstream targets of Wnt signalling.
      Results: Elevated hydrostatic pressure promoted migration and invasion of ameloblastoma cells, but inhibited proliferation. Expression of MMP-2, MMP-9, LEF-1, cyclin D1, c-Jun and c-Myc was significantly upregulated under elevated hydrostatic pressure, and these effects could be abolished by DKK1. Expression of RANKL, which is thought to be a downstream target of Wnt signalling, did not significantly change under elevated hydrostatic pressure.
      Conclusions: This study indicates that elevated hydrostatic pressure promotes the migration and invasion of ameloblastoma cells by activating the Wnt/β-catenin pathway, thereby increasing expression of MMP-2, MMP-9 and other Wnt signalling downstream targets. This suggests that marsupialization may reduce invasiveness and reverse the bone resorption process by lowering intracystic hydrostatic pressure in cystic ameloblastoma.
      (© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
    • Grant Information:
      81372884 National Natural Science Foundation of China; 81672679 National Natural Science Foundation of China
    • Contributed Indexing:
      Keywords: MMP-2; MMP-9; Wnt/β-catenin signalling pathway; ameloblastoma; hydrostatic pressure
    • Accession Number:
      0 (beta Catenin)
      EC 3.4.24.24 (Matrix Metalloproteinase 2)
      EC 3.4.24.35 (Matrix Metalloproteinase 9)
    • Publication Date:
      Date Created: 20180703 Date Completed: 20190306 Latest Revision: 20190306
    • Publication Date:
      20221213
    • Accession Number:
      10.1111/jop.12761
    • Accession Number:
      29964338