[Effect of saffron aqueous extract on the level of blood glucose in experimental diabetes mice].

Publisher: Zhongguo ying yong sheng li xue za zhi bian ji bu Country of Publication: China NLM ID: 9426407 Publication Model: Print Cited Medium: Print ISSN: 1000-6834 (Print) Linking ISSN: 10006834 NLM ISO Abbreviation: Zhongguo Ying Yong Sheng Li Xue Za Zhi Subsets: MEDLINE

Publication: Tianjin : Zhongguo ying yong sheng li xue za zhi bian ji bu
Original Publication: Beijing Shi : Zhongguo ying yong sheng li xue za zhi bian ji bu,

Blood Glucose*; Crocus/*chemistry ; Diabetes Mellitus, Experimental/*drug therapy ; Hypoglycemic Agents/*pharmacology ; Plant Extracts/*pharmacology; Animals ; Diabetes Mellitus, Experimental/chemically induced ; Insulin/blood ; Mice ; Random Allocation ; Streptozocin

Objectives: To investigate the effects of saffron aqueous extract (SE) on blood glucose, lipid and pancreatic tissue in streptozocin-induced diabetes mice.
Methods: Diabetes mellitus mice were established by intraperitoneal injection of streptozocin (60 mg/kg) for two consecutive days. The 30 well-established diabetes mice were randomly divided into three groups( n =10):diabetic mellitus (DM) group, SE treated (SE) group and positive control (metformin hydrochloride, MH) group. Another ten normal mice were selected as normal control (NC) group. The mice in SE and MH groups were intragastrically administered with SE 100 mg/kg or MH 100 mg/kg once a day for 6 weeks, mice in DM and NC were given normal saline. The amount of food-intake, water consumption and body weight were measured weekly, the changes of the indicators including fasting blood glucose (FBG), oral glucose-tolerance test (OGTT), glycated serum protein (GSP), insulin (INS) and blood lipid were determined after 6 weeks of continuous administration. The pathologic changes in the pancreas tissues were detected by HE staining.
Results: Compared with the normal control group, the amount of food-intake, water consumption, area under the curve, FBG, GSP, and total cholesterol (TC) were significantly increased, while fasting weight, INS and high density lipoprotein cholesterol (HDL-c) were dramatically decreased in DM group. Compared with DM group, the water consumption, FBG, area under the curve and TC in SE group were starkly declined with a notable elevation of HDL-c and INS. In addition, the biopsy from DM mice showed the structure of pancreas islet was destroyed and reduced, and vascular proliferation with irregular shape. The damaged pancreas was obviously repaired by giving SE.
Conclusions: The saffron aqueous extract had efficacy on blood glucose and blood lipids reduction, improvement on damaged pancreas in streptozocininduced diabetic mice, which suggested that saffron could be used for the treatment in diabetes.

Keywords: blood glucose; blood lipid; diabetes mellitus; mice; saffron

0 (Blood Glucose)
0 (Hypoglycemic Agents)
0 (Insulin)
0 (Plant Extracts)
5W494URQ81 (Streptozocin)

Date Created: 20180622 Date Completed: 20190109 Latest Revision: 20190109

20221213

10.12047/j.cjap.5588.2018.042

29926685