Item request has been placed!
×
Item request cannot be made.
×
Processing Request
Identification of immunogenic proteins and evaluation of recombinant PDHA1 and GAPDH as potential vaccine candidates against Streptococcus iniae infection in flounder (Paralichthys olivaceus).
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- Additional Information
- Source:
Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
- Publication Information:
Original Publication: San Francisco, CA : Public Library of Science
- Subject Terms:
- Abstract:
Streptococcus iniae is a major Gram-positive pathogen that causes invasive disease in fish worldwide. In this study, in order to identify immunogenic proteins for developing highly effective vaccine against S. iniae, whole-cell lysate proteins of S. iniae were analyzed by western blotting using flounder anti-S. iniae antibodies, and two positive protein bands of molecular weight 37 kDa and 40 kDa were screened, which were identified as pyruvate dehydrogenase E1 subunit alpha (PDHA1), BMP family ABC transporter substrate-binding protein (BMP) and L-lactate dehydrogenase (LDH), as well as ornithine carbamoyltransferase (OCT), lactate oxidas (LOx) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by mass spectrometry. Subsequently, the six recombinant proteins were produced and used to immunize healthy flounder, and the relative percent survival (RPS) value was 72.73%, 27.27%, 36.36%, 9.09%, 36.36% and 63.64% respectively after intraperitoneal challenge with live S. iniae, revealing that rPDHA1 and rGAPDH produced higher relative percent survival than formalin-killed S. iniae (36.36%). To further investigate the protective efficacy of rPDHA1 and rGAPDH, the proliferation of surface membrane immunoglobulin-positive (sIg+) lymphocytes in peripheral blood leucocytes, the total serum IgM, specific IgM against S. iniae and RPS were detected. The results showed that rPDHA1, rGAPDH and formalin-killed S. iniae significantly induced the proliferation of sIg+ lymphocytes, the production of total serum IgM and specific IgM as compared with the control group, and rGAPDH and rPDHA1 provide higher RPS (62.5% and 75%, respectively) again. These results demonstrated that rPDHA1 and rGAPDH are promising vaccine candidates against S. iniae infection in flounder.
Competing Interests: The authors have declared that no competing interests exist.
- References:
Emerg Infect Dis. 2009 Dec;15(12):1891-6. (PMID: 19961667)
J Clin Microbiol. 2005 Feb;43(2):933-7. (PMID: 15695711)
PLoS One. 2008 Jul 30;3(7):e2824. (PMID: 18665241)
Vaccine. 2010 May 21;28(23):3905-12. (PMID: 20394724)
J Fish Dis. 2010 Nov;33(11):901-5. (PMID: 21504081)
Appl Environ Microbiol. 2005 Sep;71(9):5654-8. (PMID: 16151172)
Vet Immunol Immunopathol. 2016 Feb;170:25-9. (PMID: 26872628)
Am J Vet Res. 2001 Feb;62(2):174-7. (PMID: 11212023)
Fish Shellfish Immunol. 2008 Jul;25(1-2):1-18. (PMID: 18448358)
Vaccine. 2010 Mar 19;28(14):2636-41. (PMID: 20096393)
Fish Shellfish Immunol. 2007 Nov;23(5):982-90. (PMID: 17719797)
Vaccine. 2009 May 11;27(21):2733-40. (PMID: 19428886)
Adv Drug Deliv Rev. 2000 Sep 15;43(1):29-43. (PMID: 10967219)
Vaccine. 2014 Oct 21;32(46):6115-21. (PMID: 25236587)
Fish Shellfish Immunol. 2015 Aug;45(2):437-42. (PMID: 25959574)
Microb Pathog. 2017 Jun;107:155-163. (PMID: 28351709)
Vaccine. 2011 Jan 10;29(3):391-4. (PMID: 21111784)
Vet Immunol Immunopathol. 1997 May;56(1-2):175-83. (PMID: 9220590)
Int J Mol Sci. 2016 Jul 12;17(7):. (PMID: 27420049)
Vet Microbiol. 2011 Sep 28;152(3-4):346-52. (PMID: 21601381)
Fish Shellfish Immunol. 2013 Sep;35(3):653-60. (PMID: 23732846)
J Proteomics. 2012 Feb 2;75(4):1263-75. (PMID: 22137900)
Microb Pathog. 2017 Dec;113:152-159. (PMID: 29074429)
FEMS Microbiol Lett. 2009 Apr;293(1):20-7. (PMID: 19278524)
Vaccine. 2016 Feb 17;34(8):1012-7. (PMID: 26686572)
Infect Immun. 2005 Oct;73(10):6935-44. (PMID: 16177373)
Fish Shellfish Immunol. 2014 Dec;41(2):250-9. (PMID: 25218275)
Fish Shellfish Immunol. 2012 Mar;32(3):391-5. (PMID: 22178783)
Vet Immunol Immunopathol. 2017 Mar;185:38-47. (PMID: 28242001)
Parasitology. 2004 Jun;128(Pt 6):603-16. (PMID: 15206462)
Fish Shellfish Immunol. 2013 Dec;35(6):1740-50. (PMID: 23507338)
Dis Aquat Organ. 2015 Oct 16;116(2):111-20. (PMID: 26480914)
Vaccine. 2014 Dec 5;32(51):7025-7032. (PMID: 25446833)
Expert Rev Vaccines. 2005 Feb;4(1):89-101. (PMID: 15757476)
Vaccine. 2017 May 31;35(24):3196-3203. (PMID: 28483195)
Exp Parasitol. 2010 Oct;126(2):224-31. (PMID: 20566413)
Vet Microbiol. 2007 May 16;122(1-2):1-15. (PMID: 17418985)
Vet Microbiol. 2013 Feb 22;162(1):151-9. (PMID: 22989514)
Int J Mol Sci. 2015 Nov 25;16(12):28001-13. (PMID: 26602918)
- Accession Number:
0 (Antibodies, Bacterial)
0 (Recombinant Proteins)
0 (Streptococcal Vaccines)
EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
EC 1.2.4.1 (Pyruvate Dehydrogenase (Lipoamide))
EC 1.2.4.1 (pyruvate dehydrogenase E1alpha subunit)
- Publication Date:
Date Created: 20180531 Date Completed: 20180806 Latest Revision: 20241108
- Publication Date:
20241108
- Accession Number:
PMC5976140
- Accession Number:
10.1371/journal.pone.0195450
- Accession Number:
29847601
No Comments.