Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.

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  • Additional Information
    • Source:
      Publisher: Wiley Country of Publication: Australia NLM ID: 100956805 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1740-0929 (Electronic) Linking ISSN: 13443941 NLM ISO Abbreviation: Anim Sci J Subsets: MEDLINE
    • Publication Information:
      Publication: Richmond, Vic. : Wiley
      Original Publication: Tokyo, Japan : Japanese Society of Zootechnical Science [1999-
    • Subject Terms:
      Electroporation* ; Embryo, Mammalian*; Cell Culture Techniques/*methods ; Cell Culture Techniques/*veterinary ; Chlorogenic Acid/*pharmacology ; Embryonic Development/*drug effects ; Fertilization in Vitro/*veterinary ; In Vitro Oocyte Maturation Techniques/*methods ; In Vitro Oocyte Maturation Techniques/*veterinary ; Oocytes/*growth & development; Animals ; Apoptosis/drug effects ; Blastocyst ; Cells, Cultured ; Dose-Response Relationship, Drug ; Stimulation, Chemical ; Swine
    • Abstract:
      Electroporation is the technique of choice to introduce an exogenous gene into embryos for transgenic animal production. Although this technique is practical and effective, embryonic damage caused by electroporation treatment remains a major problem. This study was conducted to evaluate the optimal culture system for electroporation-treated porcine embryos by supplementation of chlorogenic acid (CGA), a potent antioxidant, during in vitro oocyte maturation. The oocytes were treated with various concentrations of CGA (0, 10, 50, and 100 μmol/L) through the duration of maturation for 44 hr. The treated oocytes were then fertilized, electroporated at 30 V/mm with five 1 msec unipolar pulses, and subsequently cultured in vitro until development into the blastocyst stage. Without electroporation, the treatment with 50 μmol/L CGA had useful effects on the maturation rate of oocytes, the total cell number, and the apoptotic nucleus indices of blastocysts. When the oocytes were electroporated after in vitro fertilization, the treatment with 50 μmol/L CGA supplementation significantly improved the rate of oocytes that developed into blastocysts and reduced the apoptotic nucleus indices (4.7% and 7.6, respectively) compared with those of the untreated group (1.4% and 13.0, respectively). These results suggested that supplementation with 50 μmol/L CGA during maturation improves porcine embryonic development and quality of electroporation-treated embryos.
      (© 2018 Japanese Society of Animal Science.)
    • Contributed Indexing:
      Keywords: antioxidant; chlorogenic acid; electroporation; embryo; oxidative stress
    • Accession Number:
      318ADP12RI (Chlorogenic Acid)
    • Publication Date:
      Date Created: 20180529 Date Completed: 20181114 Latest Revision: 20181114
    • Publication Date:
      20240829
    • Accession Number:
      10.1111/asj.13049
    • Accession Number:
      29806122