HIV-1-Induced miR-146a Attenuates Monocyte Migration by Targeting CCL5 in Human Primary Macrophages.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 8709376 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1931-8405 (Electronic) Linking ISSN: 08892229 NLM ISO Abbreviation: AIDS Res Hum Retroviruses Subsets: MEDLINE
    • Publication Information:
      Publication: Larchmont, NY : Mary Ann Liebert
      Original Publication: [New York] : Mary Ann Liebert, [c1987-
    • Subject Terms:
    • Abstract:
      MicroRNAs (miRNAs) are widely involved in immune regulation during virus infection. Several studies showed that the expression of miR-146a was increased in human immunodeficiency virus type I (HIV-1)-infected cells, but the definitive function of miR-146a in HIV-1 infection remains obscure. The production of chemokine (C-C motif) ligand 5 (CCL5) in macrophages has been reported to play an important role in HIV/AIDS-associated pathogenesis. In this study, we examined the effects of miR-146a on CCL5 regulation in HIV-1-infected macrophages. Gain and loss of function studies showed that CCL5 might be one of the miR-146a targets, as miR-146a mimic reduced, while miR-146a inhibitor increased CCL5 production in HIV-1-infected macrophages. In addition, we demonstrated that miR-146a reduced CCL5-induced monocyte migration. Our study provided evidence that miR-146a targets CCL5 3' untranslated regions, downregulates its release from macrophages, and affects monocyte migration consequently. These findings drew a novel layer of posttranscriptional control of the chemokine CCL5 by miR-146a during HIV infection, which might contribute to HIV pathogenesis.
    • Contributed Indexing:
      Keywords: CCL5/RANTES; HIV-1; macrophage; miR-146a; monocyte migration
    • Accession Number:
      0 (CCL5 protein, human)
      0 (Chemokine CCL5)
      0 (MIRN146 microRNA, human)
      0 (MicroRNAs)
    • Publication Date:
      Date Created: 20180503 Date Completed: 20190722 Latest Revision: 20190722
    • Publication Date:
      20231215
    • Accession Number:
      10.1089/AID.2017.0217
    • Accession Number:
      29717615