Villous explants from preeclamptic placentas induce sFlt1 in PBMCs: An ex vivo co-culture study.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101552483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2210-7797 (Electronic) Linking ISSN: 22107789 NLM ISO Abbreviation: Pregnancy Hypertens Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amtserdam : Elsevier
    • Subject Terms:
      Cell Communication*; Chorionic Villi/*metabolism ; Leukocytes, Mononuclear/*metabolism ; Pre-Eclampsia/*metabolism ; Vascular Endothelial Growth Factor Receptor-1/*metabolism; Adult ; Case-Control Studies ; Chorionic Villi/pathology ; Coculture Techniques ; Female ; Humans ; Pre-Eclampsia/blood ; Pre-Eclampsia/genetics ; Pre-Eclampsia/pathology ; Pregnancy ; Tissue Culture Techniques ; Up-Regulation ; Vascular Endothelial Growth Factor Receptor-1/blood ; Vascular Endothelial Growth Factor Receptor-1/genetics
    • Abstract:
      Objectives: Soluble Flt1 (sFlt1) is an anti-angiogenic protein linked to the pathology of preeclampsia (PE). While the placenta serves as the major organ producing sFlt1 during normal pregnancy, peripheral blood mononuclear cells (PBMCs), endothelial cells, and stromal cells also produce sFlt1. The key question is 'what drives the overexpression of sFlt1 observed during PE?' In the present work we show evidence for sFlt1 over-expression in PBMCs due to interaction with placental villi from PE patients.
      Study Design: sFlt1 production by PBMCs is estimated by using two blood collection methods with different coagulation chemistry. PBMCs were then cultured with homologous villous explants and heterologous villous explants to determine the effects of the interaction between the two tissues.
      Main Outcome Measures: sFlt1 levels were estimated using real time PCR, ELISA, and gel electrophoresis.
      Results: Plasma samples obtained using CTAD as anti-coagulant showed 16-23% less sFlt1 compared to plasma collected in EDTA. Preeclamptic PBMCs showed higher basal level of sFlt1 mRNA. In addition, we show evidence of placental interaction as a cause of sFlt1 overexpression in PBMCs using homologous and heterologous co-culture system. However, during co-culture, we observed that while the sFlt1 expression in PE PBMCs is increased, PE villous explants show reduced sFlt1 RNA expression.
      Conclusion: sFlt1 was produced in significant amounts by preeclamptic PBMCs, and ex vivo studies show that the placenta induces this over-expression. In contrast, exposure to PBMCs appears to decrease sFlt1 production by preeclamptic placenta.
      (Copyright © 2018 International Society for the Study of Hypertension in Pregnancy. Published by Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Co-culture; PBMCs; Placental villous explants; Preeclampsia; sFlt1
    • Accession Number:
      EC 2.7.10.1 (FLT1 protein, human)
      EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
    • Publication Date:
      Date Created: 20180421 Date Completed: 20181023 Latest Revision: 20181023
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.preghy.2018.02.006
    • Accession Number:
      29674197