Fine Mapping the Interaction Between Dendritic Cell-Specific Intercellular Adhesion Molecule (ICAM)-3-Grabbing Nonintegrin and the Cytomegalovirus Envelope Glycoprotein B.

Publisher: Oxford University Press Country of Publication: United States NLM ID: 0413675 Publication Model: Print Cited Medium: Internet ISSN: 1537-6613 (Electronic) Linking ISSN: 00221899 NLM ISO Abbreviation: J Infect Dis Subsets: MEDLINE

Publication: Jan. 2011- : Oxford : Oxford University Press
Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press

Host-Pathogen Interactions*; Cell Adhesion Molecules/*metabolism ; Cytomegalovirus/*physiology ; Dendritic Cells/*virology ; Lectins, C-Type/*metabolism ; Receptors, Cell Surface/*metabolism ; Receptors, Virus/*metabolism ; Viral Envelope Proteins/*metabolism; Cell Adhesion Molecules/genetics ; Cells, Cultured ; DNA Mutational Analysis ; Humans ; Lectins, C-Type/genetics ; Mutant Proteins/genetics ; Mutant Proteins/metabolism ; Polysaccharides/metabolism ; Protein Binding ; Protein Interaction Mapping ; Receptors, Cell Surface/genetics ; Receptors, Virus/genetics ; Viral Envelope Proteins/genetics ; Virus Attachment

Background: Human cytomegalovirus (HCMV) is a leading cause of virally induced congenital disorders and morbidities in immunocompromised individuals, ie, transplant, cancer, or acquired immune deficiency syndrome patients. Human cytomegalovirus infects virtually all cell types through the envelope glycoprotein complex gH/gL/gO with or without a contribution of the pentameric gH/gL/pUL128L. Together with gH/gL, the HCMV envelope glycoprotein B (gB) contributes to the viral fusion machinery.
Methods: We previously showed that gB is a ligand for the C-type lectin dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) contributing to HCMV attachment to and infection of DC-SIGN-expressing cells. However, the features of the DC-SIGN/gB interaction remain unclear. To address this point, the role of glycans on gB and the consequences of mutagenesis and antibody-mediated blockades on both partners were examined in this study.
Results: We identified DC-SIGN amino acid residues involved in this interaction through an extensive mutagenesis study. We also showed the importance of high-mannose N-glycans decorating the asparagine residue at position 208, demonstrating that the antigenic domain 5 on gB is involved in the interaction with DC-SIGN. Finally, antibody-mediated blockades allowed us to identify DC-SIGN as a major HCMV attachment receptor on monocyte-derived dendritic cells.
Conclusions: Taken together, these results have permitted us to fine-map the interaction between DC-SIGN and HCMV gB.

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0 (Cell Adhesion Molecules)
0 (DC-specific ICAM-3 grabbing nonintegrin)
0 (Lectins, C-Type)
0 (Mutant Proteins)
0 (Polysaccharides)
0 (Receptors, Cell Surface)
0 (Receptors, Virus)
0 (Viral Envelope Proteins)
0 (glycoprotein B, Simplexvirus)

Date Created: 20180413 Date Completed: 20190912 Latest Revision: 20190912

20231215

PMC6049025

10.1093/infdis/jiy194

29648611