Characteristic glycopeptides associated with extreme human longevity identified through plasma glycoproteomics.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731726 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0304-4165 (Print) Linking ISSN: 03044165 NLM ISO Abbreviation: Biochim Biophys Acta Gen Subj Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier
    • Subject Terms:
      Biomarkers/*blood ; Blood Proteins/*metabolism ; Glycopeptides/*blood ; Glycoproteins/*blood ; Longevity/*physiology ; Polysaccharides/*blood ; Proteomics/*methods; Adult ; Age Factors ; Aged ; Aged, 80 and over ; Case-Control Studies ; Female ; Glycosylation ; Humans ; Young Adult
    • Abstract:
      Background: Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity. Semi-supercentenarians (SSCs; older than 105 years) are thought to be a model of human longevity. Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity.
      Methods: Plasma proteins from Japanese semi-supercentenarians (SSCs, 106-109 years), aged controls (70-88 years), and young controls (20-38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS). Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA). Furthermore, plasma levels of clinical biomarkers were measured.
      Results: We found two lectins such as Phaseolus vulgaris, and Erythrina cristagalli (ECA), of which protein binding were characteristically increased in SSCs. Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs. Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis. However, the SSCs analysed here did not suffer from these diseases.
      Conclusions: Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.
      General Significance: We found abundant glycans in SSCs, which may be associated with human longevity.
      (Copyright © 2018 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: LC/MS; Lectin microarray; Longevity; N-glycosylation; Semi-supercentenarian
    • Accession Number:
      0 (Biomarkers)
      0 (Blood Proteins)
      0 (Glycopeptides)
      0 (Glycoproteins)
      0 (Polysaccharides)
    • Publication Date:
      Date Created: 20180328 Date Completed: 20181106 Latest Revision: 20181106
    • Publication Date:
      20240628
    • Accession Number:
      10.1016/j.bbagen.2018.03.025
    • Accession Number:
      29580922