Proteases and nucleases involved in the biphasic digestion process of the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae).

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  • Author(s): Lomate PR;Lomate PR; Bonning BC; Bonning BC; Bonning BC
  • Source:
    Archives of insect biochemistry and physiology [Arch Insect Biochem Physiol] 2018 Jul; Vol. 98 (3), pp. e21459. Date of Electronic Publication: 2018 Mar 12.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Wiley Country of Publication: United States NLM ID: 8501752 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6327 (Electronic) Linking ISSN: 07394462 NLM ISO Abbreviation: Arch Insect Biochem Physiol Subsets: MEDLINE
    • Publication Information:
      Publication: New York, NY : Wiley
      Original Publication: New York : Alan R. Liss, c1983-
    • Subject Terms:
      Digestion*; Deoxyribonucleases/*metabolism ; Hemiptera/*enzymology ; Peptide Hydrolases/*metabolism ; Ribonucleases/*metabolism; Animals ; Substrate Specificity
    • Abstract:
      Management of the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), an invasive, agricultural pest in the United States, has presented significant challenges. This polyphagous insect uses both extra-oral and gut-based digestion thwarting protein- or nucleotide-based control strategies. The objective of this study was to biochemically characterize the digestive enzymes (proteases and nucleases) from the saliva, salivary gland and the gut of H. halys. Enzyme profiles for the two tissues and saliva radically differ: The pH optimum for proteases in the gut was six, with cysteine proteases predominant. In contrast, the alkaline pH optima for protease activity in the salivary gland (8-10) and saliva (7) reflected abundant serine protease and cathepsin activities. RNase enzymes were most abundant in saliva, while dsRNase and DNase activities were higher in the salivary gland and saliva compared to those in the gut. These very different enzyme profiles highlight the biphasic digestive system used by this invasive species for efficient processing of plant nutrients. Knowledge of H. halys digestive physiology will allow for counteractive measures targeting digestive enzymes or for appropriate protection of protein- or nucleotide-based management options targeting this pest.
      (© 2018 Wiley Periodicals, Inc.)
    • Contributed Indexing:
      Keywords: Halyomorpha halys; gut; nuclease; protease; saliva
    • Accession Number:
      EC 3.1.- (Deoxyribonucleases)
      EC 3.1.- (Ribonucleases)
      EC 3.4.- (Peptide Hydrolases)
    • Publication Date:
      Date Created: 20180313 Date Completed: 20180709 Latest Revision: 20180709
    • Publication Date:
      20240829
    • Accession Number:
      10.1002/arch.21459
    • Accession Number:
      29527721