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Sensitive method for glycosaminoglycan analysis of tissue sections.
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- Additional Information
- Source:
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3778 (Electronic) Linking ISSN: 00219673 NLM ISO Abbreviation: J Chromatogr A Subsets: MEDLINE
- Publication Information:
Original Publication: Amsterdam ; New York : Elsevier, 1993-
- Subject Terms:
- Abstract:
A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC-MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10 μg).
(Copyright © 2018 Elsevier B.V. All rights reserved.)
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- Grant Information:
P41 GM104603 United States GM NIGMS NIH HHS; U01 CA221234 United States CA NCI NIH HHS
- Contributed Indexing:
Keywords: Glycosaminoglycan; Heparan sulfate; Hydrophilic interaction chromatography; Mass spectrometry; Prostate cancer; Tissue microarray
- Accession Number:
0 (Disaccharides)
0 (Glycosaminoglycans)
9050-30-0 (Heparitin Sulfate)
- Publication Date:
Date Created: 20180307 Date Completed: 20180514 Latest Revision: 20200225
- Publication Date:
20221213
- Accession Number:
PMC6590710
- Accession Number:
10.1016/j.chroma.2018.02.034
- Accession Number:
29506752
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