rApoptin induces apoptosis in human breast cancer cells via phosphorylation of Nur77 and Akt.

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  • Author(s): Hou Z;Hou Z; Mao J; Mao J; Mao J; Mao J; Lu Y; Lu Y; Lu Y; Li L; Li L; Li L
  • Source:
    Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2018 Mar 25; Vol. 498 (1), pp. 221-227. Date of Electronic Publication: 2018 Mar 01.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
    • Publication Information:
      Publication: <2002- >: San Diego, CA : Elsevier
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Apoptosis/*drug effects ; Breast Neoplasms/*metabolism ; Breast Neoplasms/*pathology ; Capsid Proteins/*pharmacology ; Nuclear Receptor Subfamily 4, Group A, Member 1/*metabolism ; Proto-Oncogene Proteins c-akt/*metabolism ; Recombinant Proteins/*pharmacology; Animals ; Carcinogenesis/pathology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Female ; Humans ; Mice, Nude ; Phosphorylation/drug effects
    • Abstract:
      Breast cancer is the leading cause of cancer incidence and cancer-related mortality among women and is becoming a major public health problem around the world. The current study aims to investigate the possible role and mechanism of recombinant Apoptin (rApoptin), a potential anticancer candidate that minimally impacts normal cells, in the breast cancer cell proliferation and apoptosis in vitro and in vivo. We found that rApoptin could effectively inhibit the proliferation and apoptosis in MCF-7 and MDA-MB-231 cells in vitro, which was further confirmed by flow cytometry analysis. Apoptin partially inhibited MCF-7 cell xenograft tumor development in vivo. Furthermore, we found via western blot that rApoptin-induced apoptosis in MCF-7 and MDA-MB-231 cells was associated with the phosphorylation of Nur77 (p-Nur77) and Akt (p-Akt). In addition, compared with the control groups, rApoptin-treated tissues showed significantly higher expression of Bax and Cyt c while Bcl-2 expression was decreased by rApoptin treatment. Together, our results are the first to demonstrate that rApoptin was able to effectively induce breast cancer cell apoptosis both in vitro and in vivo and that this activity could be regulated by the phosphorylation of Nur77 and Akt and the mitochondrial pathway. Our findings highlight the potential application of rApoptin as a breast cancer treatment.
      (Copyright © 2018 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Akt; Apoptosis; Breast cancer; Nur77; rApoptin
    • Accession Number:
      0 (Capsid Proteins)
      0 (Nuclear Receptor Subfamily 4, Group A, Member 1)
      0 (Recombinant Proteins)
      0 (VP3 protein, Chicken anemia virus)
      EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
    • Publication Date:
      Date Created: 20180305 Date Completed: 20180406 Latest Revision: 20180406
    • Publication Date:
      20240628
    • Accession Number:
      10.1016/j.bbrc.2018.02.204
    • Accession Number:
      29501489