The therapeutic potential of cystathionine gamma-lyase in temporomandibular inflammation-induced orofacial hypernociception.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: United States NLM ID: 0151504 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-507X (Electronic) Linking ISSN: 00319384 NLM ISO Abbreviation: Physiol Behav Subsets: MEDLINE
    • Publication Information:
      Publication: New York NY : Elsevier Science
      Original Publication: Oxford, Eng., Long Island City, Pergamon Press.
    • Subject Terms:
      Cystathionine gamma-Lyase/*metabolism ; Facial Pain/*enzymology ; Facial Pain/*etiology ; Inflammation/*complications ; Inflammation/*pathology ; Temporomandibular Joint/*pathology; Alkynes/therapeutic use ; Analysis of Variance ; Animals ; Enzyme Inhibitors/therapeutic use ; Facial Pain/complications ; Facial Pain/drug therapy ; Freund's Adjuvant/toxicity ; Glycine/analogs & derivatives ; Glycine/therapeutic use ; Hyperalgesia/drug therapy ; Hyperalgesia/etiology ; Inflammation/chemically induced ; Male ; Pain Measurement ; Rats ; Rats, Wistar ; Time Factors ; Treatment Outcome
    • Abstract:
      Hydrogen sulfide (H 2 S) is an endogenous neuromodulator produced mainly by the enzyme cystathionine gamma-lyase (CSE) in peripheral tissues. A pronociceptive role of endogenously produced H 2 S has been previously reported by our group in a model of orofacial inflammatory pain. Using the established persistent orofacial pain rat model induced by complete Freund's adjuvant (CFA) injection into temporomandibular joint (TMJ), we have now investigated the putative role of endogenous H 2 S modulating hypernociceptive responses. Additionally, plasmatic extravasation on TMJ was measured following different treatments by Evans blue dye quantification. Thus, rats were submitted to Von Frey and Formalin tests in orofacial region before and after pharmacological inhibition of the CSE-H 2 S system combined or not with CFA-induced TMJ inflammation. Pretreatment with CSE inhibitor, propargylglycine (PAG; 88.4 μmol/kg) reduced temporomandibular inflammatory pain when injected locally as well as systemically. In particular, local PAG injection seems to be more effective for hypernociceptive responses in orofacial persistent inflammation since its action is evidenced in the majority analyzed periods of the inflammatory process compared to its systemic use. Moreover, local injection seems to act on temporomandibular vascular permeability, evidenced by decreased plasmatic extravasation induced by local PAG administration. Our data are consistent with the notion that the endogenous synthetized gas H 2 S modulates persistent orofacial pain responses revealing the pharmacological importance of the CSE inhibitor as a possible therapeutic target for their control.
      (Copyright © 2018 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Cystathionine gamma-lyase; Hydrogen sulfide; Pain; Temporomandibular joint inflammation
    • Accession Number:
      0 (Alkynes)
      0 (Enzyme Inhibitors)
      64165-64-6 (propargylglycine)
      9007-81-2 (Freund's Adjuvant)
      EC 4.4.1.1 (Cystathionine gamma-Lyase)
      TE7660XO1C (Glycine)
    • Publication Date:
      Date Created: 20180210 Date Completed: 20190131 Latest Revision: 20190131
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.physbeh.2018.02.007
    • Accession Number:
      29425970