Effect of superstimulation on the expression of microRNAs and genes involved in steroidogenesis and ovulation in Nelore cows.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE
    • Publication Information:
      Publication: [New York, N.Y.?] : Elsevier
      Original Publication: Los Altos, Calif., Geron-X.
    • Subject Terms:
      Cattle/*genetics ; Gonadal Steroid Hormones/*biosynthesis ; MicroRNAs/*genetics ; Ovulation/*genetics ; Superovulation/*genetics; Animals ; Estrus Synchronization/physiology ; Female ; Gene Expression ; Metabolic Networks and Pathways/genetics ; Ovulation Induction/methods ; Ovulation Induction/veterinary ; Superovulation/physiology
    • Abstract:
      To better understand the impact of ovarian superstimulation on bovine follicular microenvironment, Nelore cows (Bos taurus indicus) were subjected to ovarian superstimulation with follicle stimulating hormone (FSH, n = 10; P-36 protocol) or FSH combined with eCG (n = 10; P-36/eCG protocol). Follicular fluid was analyzed for cholesterol concentration. Granulosa cells were analyzed by RT-qPCR to assess the expression of genes involved in steroidogenic and ovulatory and expression of microRNAs involved in final follicular development and luteinizing hormone/choriogonadotropin receptor (LHCGR) expression. Plasma concentration of estradiol was also measured. Follicular fluid from the P-36 group showed higher concentration of cholesterol than that of control (non-superstimulated) cows. Plasma concentration of estradiol was higher in the P-36/eCG group. Abundance of STAR and FSHR mRNAs were lower in granulosa cells from the P-36/eCG group. In contrast, LHCGR mRNA abundance was higher in superstimulated granulosa cells from the P-36 group and showed a pattern opposite to that of miR-222 expression. Ovarian superstimulation did not affect the expression of other markers (mmu-miR-202-5p, has-miR-873, has-miR-144, and their target genes, CREB, TGFBR2, and ATG7) of antral follicle development. However, the mRNA expression of VEGF pathway components was modulated by P-36 treatment. Taken together, these results demonstrate that superstimulatory protocols modify steroidogenic capacity, increase plasma estradiol, and regulate the abundance of VEGF system, LHCGR mRNA and suppress the expression of miR-222 in bovine granulosa cells.
      (Copyright © 2017. Published by Elsevier Inc.)
    • Accession Number:
      0 (Gonadal Steroid Hormones)
      0 (MicroRNAs)
    • Publication Date:
      Date Created: 20180207 Date Completed: 20180924 Latest Revision: 20180924
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.theriogenology.2017.12.045
    • Accession Number:
      29407901