Serum effect on cellular uptake of spermidine, spergualin, 15-deoxyspergualin, and their metabolites by L5178Y cells.

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  • Author(s): Kunimoto S;Kunimoto S; Nosaka C; Xu CZ; Takeuchi T
  • Source:
    The Journal of antibiotics [J Antibiot (Tokyo)] 1989 Jan; Vol. 42 (1), pp. 116-22.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0151115 Publication Model: Print Cited Medium: Print ISSN: 0021-8820 (Print) Linking ISSN: 00218820 NLM ISO Abbreviation: J Antibiot (Tokyo) Subsets: MEDLINE
    • Publication Information:
      Publication: 2009- : London : Nature Publishing Group
      Original Publication: Tokyo, Japan Antibiotics Research Assn.
    • Subject Terms:
      Amine Oxidase (Copper-Containing)*; Oxidoreductases Acting on CH-NH Group Donors/*physiology ; Spermidine/*pharmacokinetics; Animals ; Cell Survival/drug effects ; Guanidines/pharmacokinetics ; Guanidines/pharmacology ; Lymphoma, Non-Hodgkin/metabolism ; Mice ; Oxidoreductases Acting on CH-NH Group Donors/antagonists & inhibitors ; Oxidoreductases Acting on CH-NH Group Donors/blood ; Tumor Cells, Cultured
    • Abstract:
      Spergualin (SG) and 15-deoxyspergualin (DSG) were more slowly incorporated into L5178Y cells than spermidine. SG and DSG inhibited carrier-mediated transport of [3H]spermidine competitively with inhibition constants of 0.67 mM and 0.45 mM, respectively. Addition of calf serum stimulated uptake of [3H]spermidine into the cells in a serum concentration-dependent manner. The effect was not observed when horse serum was used in place of calf serum. Preincubation of spermidine in calf serum for 1 hour before addition to cells remarkably decreased cellular incorporation of tritium. Three amine oxidase inhibitors, aminoguanidine, 3-hydroxybenzyloxyamine, and semicarbazide, inhibited stimulation of uptake of [3H]spermidine by calf serum and the decrease of it by preincubation in calf serum. So we propose that cellular incorporation or binding of products generated by oxidation of spermidine by amine oxidase in calf serum was much faster than that of spermidine itself and they were unstable and transformed quickly to unincorporable or non-binding substances if cellular targets were not present. Effect of amine oxidase inhibitors on cytotoxic activity of SG and DSG were determined in low and high concentrations of calf serum. In the presence of 10% calf serum in the basal medium, cytotoxicity to L5178Y cells by SG and DSG was suppressed at high drug concentrations (above 10 micrograms/ml) and enhanced at low drug concentrations (below 2.5 micrograms/ml) by amine oxidase inhibitors. In the presence of 0.5% calf serum suppression of cytotoxicity at high drug concentrations by amine oxidase inhibitors was also observed, but enhancement at low drug concentrations was obscure. These data may suggest the existence of two kinds of cytotoxic mechanism of SG and DSG, one dependent on and one independent of amine oxidase in serum.
    • Accession Number:
      0 (Guanidines)
      71O2A541CU (spergualin)
      EC 1.4.3.21 (Amine Oxidase (Copper-Containing))
      EC 1.5.- (Oxidoreductases Acting on CH-NH Group Donors)
      U87FK77H25 (Spermidine)
      UJ0ZJ76DO9 (gusperimus)
    • Publication Date:
      Date Created: 19890101 Date Completed: 19890413 Latest Revision: 20190723
    • Publication Date:
      20231215
    • Accession Number:
      10.7164/antibiotics.42.116
    • Accession Number:
      2921216