In vitro production of 1,25-dihydroxyvitamin D3 by rat placental tissue.

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  • Author(s): Tanaka Y; Halloran B; Schnoes HK; DeLuca HF
  • Source:
    Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1979 Oct; Vol. 76 (10), pp. 5033-5.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : National Academy of Sciences
    • Subject Terms:
      Dihydroxycholecalciferols/*biosynthesis ; Hydroxycholecalciferols/*biosynthesis ; Placenta/*metabolism; Animals ; Chromatography, High Pressure Liquid ; Female ; Hydroxycholecalciferols/metabolism ; Mass Spectrometry ; Pregnancy ; Rats
    • Abstract:
      Weanling female rats were fed a vitamin D-deficient diet for 4 months until they reached maturity. They were mated with normal, vitamin D-replete male rats and, at 20 days of pregnancy, the female rats were killed and their placentae were removed, homogenzied, and incubated with 25-hydroxyvitamin D3. The incubation mixtures were extracted and the extracts were subjected to Sephadex LH-20 column chromatography followed by high-pressure liquid chromatography. The 1,25-dihydroxyvitamin D3 region of the high-pressure liquid chromatogram was recycled to purity and the structure of the product was identified as 1,25-dihydroxyvitamin D3 by ultraviolet absorption spectrophotometry and by mass spectrometry. Thus it is now evident that placenta, in addition to renal tissue, is capable of converting 25-hydroxyvitamin D3 to the hormonal form of vitamin D, 1,25-dihydroxyvitamin D3.
    • References:
      Arch Biochem Biophys. 1976 Sep;176(1):235-43. (PMID: 9906)
      Science. 1979 Jun 22;204(4399):1311-3. (PMID: 451538)
      Endocrinology. 1978 Dec;103(6):1992-6. (PMID: 748031)
      J Lipid Res. 1971 Jul;12(4):460-5. (PMID: 4362142)
      J Nutr. 1970 Sep;100(9):1049-52. (PMID: 4318536)
      Science. 1971 Jun 18;172(3989):1232-4. (PMID: 4325104)
      Biochem Biophys Res Commun. 1971 Mar 19;42(6):1082-7. (PMID: 4323845)
      Nature. 1970 Nov 21;228(5273):764-6. (PMID: 4319631)
      Proc Natl Acad Sci U S A. 1971 Apr;68(4):803-4. (PMID: 4323790)
      J Lipid Res. 1966 Nov;7(6):739-44. (PMID: 5971569)
    • Accession Number:
      0 (Dihydroxycholecalciferols)
      0 (Hydroxycholecalciferols)
    • Publication Date:
      Date Created: 19791001 Date Completed: 19800128 Latest Revision: 20190501
    • Publication Date:
      20240829
    • Accession Number:
      PMC413073
    • Accession Number:
      10.1073/pnas.76.10.5033
    • Accession Number:
      291919