Identification of Brucella genus and eight Brucella species by Luminex bead-based suspension array.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: England NLM ID: 8601127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9998 (Electronic) Linking ISSN: 07400020 NLM ISO Abbreviation: Food Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: London : Elsevier
      Original Publication: London ; Orlando : Academic Press, c1984-
    • Subject Terms:
      Bacterial Typing Techniques/*methods ; Brucella/*isolation & purification ; Brucellosis/*microbiology ; Oligonucleotide Array Sequence Analysis/*methods; Animals ; Bacterial Typing Techniques/instrumentation ; Brucella/classification ; Brucella/genetics ; Brucellosis/transmission ; DNA, Bacterial/genetics ; Humans ; Milk/microbiology ; Oligonucleotide Array Sequence Analysis/instrumentation ; Sensitivity and Specificity ; Sheep
    • Abstract:
      Globally, unpasteurized milk products are vehicles for the transmission of brucellosis, a zoonosis responsible for cases of foodborne illness in the United States and elsewhere. Existing PCR assays to detect Brucella species are restricted by the resolution of band sizes on a gel or the number of fluorescent channels in a single real-time system. The Luminex bead-based suspension array is performed in a 96-well plate allowing for high throughput screening of up to 100 targets in one sample with easily discernible results. We have developed an array using the Bio-Plex 200 to differentiate the most common Brucella species: B. abortus, B. melitensis, B. suis, B. suis bv5, B. canis, B. ovis, B. pinnipedia, and B. neotomae, as well as Brucella genus. All probes showed high specificity, with no cross-reaction with non-Brucella strains. We could detect pure DNA from B. abortus, B. melitensis, and genus-level Brucella at concentrations of ≤5 fg/μL. Pure DNA from all other species tested positive at concentrations well below 500 fg/μL and we positively identified B. neotomae in six artificially contaminated cheese and milk products. An intra-laboratory verification further demonstrated the assay's accuracy and robustness in the rapid screening (3-4 h including PCR) of DNA.
      (Published by Elsevier Ltd.)
    • Contributed Indexing:
      Keywords: Bio-plex 200; Brucella DNA detection; Brucella speciation; High-throughput
    • Accession Number:
      0 (DNA, Bacterial)
    • Publication Date:
      Date Created: 20171128 Date Completed: 20180419 Latest Revision: 20191210
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.fm.2017.09.015
    • Accession Number:
      29173617