Safety of transtympanic application of probiotics in a chinchilla animal model.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101479544 Publication Model: Electronic Cited Medium: Internet ISSN: 1916-0216 (Electronic) Linking ISSN: 19160208 NLM ISO Abbreviation: J Otolaryngol Head Neck Surg Subsets: MEDLINE
    • Publication Information:
      Publication: 2013- : London : BioMed Central
      Original Publication: Hamilton, Ont. : Decker
    • Subject Terms:
      Otitis Media, Suppurative/*drug therapy ; Probiotics/*administration & dosage ; Tympanic Membrane/*drug effects; Administration, Topical ; Animals ; Chinchilla ; Chronic Disease ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Drug Administration Schedule ; Female ; Follow-Up Studies ; Hearing Tests/methods ; Otitis Media, Suppurative/microbiology ; Random Allocation ; Reference Values ; Risk Assessment ; Treatment Outcome
    • Abstract:
      Background: Chronic suppurative otitis media can be recalcitrant and difficult to treat, particularly with the increasing occurrence of antibiotic resistance. Lactobacillus plantarum is a probiotic that has been shown to decrease S. aureus and P. aeruginosa growth in wounds, making it a good candidate for the treatment of chronic suppurative otitis media. However, before it can be applied in the ear, its ototoxicity potential must be evaluated.
      Methods: A prospective controlled trial was conducted in a chinchilla animal model at the Animal care research facilities of the Montreal Children's Hospital Research Institute to determine whether Lactobacillus plantarum is ototoxic when applied transtympanically. Ten chinchillas each had one ear randomly assigned to receive 10 9  CFU/mL of Lactobacillus plantarum solution, while the contralateral ear received saline. Auditory brainstem responses were measured bilaterally at 8, 20, 25 kHz before, at 7-10 days after application, and at 28 days after application of probiotic or saline. Facial nerve and vestibular function were assessed clinically.
      Results: There were no statistically significant differences in hearing thresholds between control and experimental ears at 28 days after application. A difference of 11 dB was noted in the 25 kHz range at day 7-10, but resolved by day 28. No animals receiving probiotics developed vestibular nerve dysfunction. There was no histologic evidence of auditory hair cell damaged evidenced by scanning electron microscopy.
      Conclusion: Our study suggests that a single application of Lactobacillus plantarum at 10 9  CFU/mL does not cause ototoxicity in a chinchilla animal model. These preliminary safety evaluations and the pathogen inhibitory effects of L. plantarum demonstrated by previous studies present this probiotic as a candidate of interest for further investigation.
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    • Contributed Indexing:
      Keywords: Chinchilla; Otitis; Ototoxicity; Probiotic; Transtympanic
    • Publication Date:
      Date Created: 20171124 Date Completed: 20180822 Latest Revision: 20181113
    • Publication Date:
      20231215
    • Accession Number:
      PMC5700520
    • Accession Number:
      10.1186/s40463-017-0242-y
    • Accession Number:
      29166927