Novel Polymerase Spiral Reaction (PSR) for rapid visual detection of Bovine Herpesvirus 1 genomic DNA from aborted bovine fetus and semen.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
    • Subject Terms:
      Aborted Fetus/*virology ; Cattle Diseases/*virology ; DNA, Viral/*genetics ; Herpesviridae Infections/*virology ; Herpesvirus 1, Bovine/*genetics ; Semen/*virology; Animals ; Cattle ; DNA Primers/genetics ; Genomics/methods ; Real-Time Polymerase Chain Reaction/methods
    • Abstract:
      Bovine herpesvirus-1 (BHV-1) is a major viral pathogen affecting bovines leading to various clinical manifestations and causes significant economic impediment in modern livestock production system. Rapid, accurate and sensitive detection of BHV-1 infection at frozen semen stations or at dairy herds remains a priority for control of BHV-1 spread to susceptible population. Polymerase Spiral Reaction (PSR), a novel addition in the gamut of isothermal techniques, has been successfully implemented in initial optimization for detection of BHV-1 genomic DNA and further validated in clinical samples. The developed PSR assay has been validated for detection of BHV-1 from bovine semen (n=99), a major source of transmission of BHV-1 from breeding bulls to susceptible dams in artificial insemination programs. The technique has also been used for screening of BHV-1 DNA from suspected aborted fetal tissues (n=25). The developed PSR technique is 100 fold more sensitive than conventional PCR and comparable to real-time PCR. The PSR technique has been successful in detecting 13 samples positive for BHV-1 DNA in bovine semen, 4 samples more than conventional PCR. The aborted fetal tissues were negative for presence of BHV-1 DNA. The presence of BHV-1 in bovine semen samples raises a pertinent concern for extensively screening of semen from breeding bulls before been used for artificial insemination process. PSR has all the attributes for becoming a method of choice for rapid, accurate and sensitive detection of BHV-1 DNA at frozen semen stations or at dairy herds in resource constrained settings.
      (Copyright © 2017 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: BHV-1 subtypes; Bovine Herpesvirus-1; Polymerase Spiral Reaction; Real-time PCR
    • Accession Number:
      0 (DNA Primers)
      0 (DNA, Viral)
    • Publication Date:
      Date Created: 20171107 Date Completed: 20180119 Latest Revision: 20180119
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.gene.2017.11.004
    • Accession Number:
      29104164