Molecular identification of Neofabraea species associated with bull's-eye rot on apple using rolling-circle amplification of partial EF-1α sequence.

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  • Additional Information
    • Source:
      Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 0372707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1480-3275 (Electronic) Linking ISSN: 00084166 NLM ISO Abbreviation: Can J Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : Ottawa, ON : Canadian Science Publishing
      Original Publication: Ottawa, Canada : National Research Council, [1954-
    • Subject Terms:
      Nucleic Acid Amplification Techniques*; Ascomycota/*genetics ; Malus/*microbiology ; Microbiological Techniques/*methods ; Peptide Elongation Factor 1/*genetics; Ascomycota/classification ; Species Specificity
    • Abstract:
      A rolling-circle amplification (RCA) method with padlock probes targeted on EF-1α regions was developed for rapid detection of apple bull's-eye rot pathogens, including Neofabraea malicorticis, N. perennans, N. kienholzii, and N. vagabunda (synonym: N. alba). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk, and PLP-Nv) were designed and tested against 28 samples, including 22 BER pathogen cultures, 4 closely related species, and 2 unrelated species that may cause serious apple decays. The assay successfully identified all the bull's-eye rot pathogenic fungi at the level of species, while no cross-reaction was observed in all target species and no false-positive reaction was observed with all strains used for reference. This study showed that the use of padlock probes and the combination of probe signal amplification by RCA provided an effective and sensitive method for the rapid identification of Neofabraea spp. The method could therefore be a useful tool for monitoring bull's-eye rot pathogens in port quarantine and orchard epidemiological studies.
    • Contributed Indexing:
      Keywords: Neofabraea; amplification circulaire; anthracnose de la pomme; bull’s-eye rot of apple; identification; padlock probe; rolling circle amplification; sonde cadenas
    • Accession Number:
      0 (Peptide Elongation Factor 1)
    • Publication Date:
      Date Created: 20171031 Date Completed: 20180403 Latest Revision: 20181202
    • Publication Date:
      20240829
    • Accession Number:
      10.1139/cjm-2017-0448
    • Accession Number:
      29084390