Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.

Arabidopsis Proteins/*genetics ; Basic Helix-Loop-Helix Transcription Factors/*genetics ; Cryptochromes/*genetics ; Gene Expression Regulation/*genetics ; Optogenetics/*methods ; Transcriptional Activation/*genetics; 3T3 Cells ; Animals ; Arabidopsis/enzymology ; Arabidopsis/genetics ; Arabidopsis Proteins/metabolism ; Basic Helix-Loop-Helix Transcription Factors/metabolism ; Cell Line ; Cryptochromes/metabolism ; Female ; HEK293 Cells ; Humans ; Light ; Male ; Mice ; Mice, Inbred C57BL ; Protein Binding/genetics

Light-inducible systems allow spatiotemporal control of a variety of biological activities. Here, we report newly optimized optogenetic tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Flavin Kelch-repeat F-box 1 (FKF1) and its binding partner GIGANTEA (GI) as well as CRY2/CIB1. By combining the mutagenesis of FKF1 with the optimization of a split FKF1/GI dimerized Gal4-VP16 transcriptional system, we identified constructs enabling significantly improved light-triggered transcriptional induction. In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization. The improvements regarding the FKF1/GI- and CRY2/CIB1-based systems will be widely applicable for the light-dependent control of transcription in mammalian cells.
(© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.)

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0 (Arabidopsis Proteins)
0 (Basic Helix-Loop-Helix Transcription Factors)
0 (CIB1 protein, Arabidopsis)
0 (CRY2 protein, Arabidopsis)
0 (Cryptochromes)
0 (FKF1 protein, Arabidopsis)
0 (GI protein, Arabidopsis)

Date Created: 20171018 Date Completed: 20171204 Latest Revision: 20181113

20221213

PMC5714181

10.1093/nar/gkx804

29040770