[In vitro and in vivo recoveries of cutaneous micro-dialysis probe of paeonol, eugenol and piperine].

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  • Additional Information
    • Source:
      Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE
    • Publication Information:
      Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo
      Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989-
    • Subject Terms:
      Microdialysis*; Acetophenones/*isolation & purification ; Alkaloids/*isolation & purification ; Benzodioxoles/*isolation & purification ; Eugenol/*isolation & purification ; Piperidines/*isolation & purification ; Polyunsaturated Alkamides/*isolation & purification; Drug Delivery Systems ; Transdermal Patch
    • Abstract:
      To establish a method for detecting micro-dialysis recovery of paeonol, eugenol and piperine in Huoxue Zhitong patch, in order to provide the basis for further percutaneous pharmacokinetics studies. The concentrations of paeonol, eugenol and piperine in dialysates were determined by HPLC, and probe deliveries were calculated respectively. The effects of concentration and calibration approaches on the micro-dialysis probe deliveries of the three components were investigated, and their probe absorbability, in vitro and in vivo probe stability and repeatability were also studied.The results indicated that little paeonol, eugenol and piperine were observed in probes with 30% alcohol as the perfusate, and could be cleaned from probe in a short time. And the in vivo and in vitro probe deliveries of three components were stable within 8 h, drug-containing solution and blank perfusate were alternatively used for three times, and the in vivo and in vitro probe deliveries of three components were basically unchanged. The in vitro recoveries of paeonol, eugenol and piperine with a range of concentration were respectively (45.7±4.66)%, (27.82±2.95)%, (41.3±3.96)%, which indicated no concentration independent. Under the same conditions, the similar delivery was observed by dialysis, retrodialysis and no-net flux. Therefore, the concentrations of analyses of the collected fraction could be calibrated by in vitro or in vivo recoveries. Meanwhile, this also proved that the micro-dialysis method built by this study is applicable to the study on percutaneous pharmacokinetics of Huoxue Zhitong patch.
      Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.
      (Copyright© by the Chinese Pharmaceutical Association.)
    • Contributed Indexing:
      Keywords: eugenol; micro-dialysis; paeonol; piperine; probe delivery
    • Accession Number:
      0 (Acetophenones)
      0 (Alkaloids)
      0 (Benzodioxoles)
      0 (Piperidines)
      0 (Polyunsaturated Alkamides)
      3R834EPI82 (paeonol)
      3T8H1794QW (Eugenol)
      U71XL721QK (piperine)
    • Publication Date:
      Date Created: 20170922 Date Completed: 20180530 Latest Revision: 20181202
    • Publication Date:
      20231215
    • Accession Number:
      10.4268/cjcmm20162226
    • Accession Number:
      28933096