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Impact of processing methods on urinary biomarkers analysis in neonates.
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- Author(s): Starr MC;Starr MC; Askenazi DJ; Askenazi DJ; Goldstein SL; Goldstein SL; MacDonald JW; MacDonald JW; Bammler TK; Bammler TK; Afsharinejad Z; Afsharinejad Z; D Brophy P; D Brophy P; Juul SE; Juul SE; Mayock DE; Mayock DE; Hingorani SR; Hingorani SR
- Source:
Pediatric nephrology (Berlin, Germany) [Pediatr Nephrol] 2018 Jan; Vol. 33 (1), pp. 181-186. Date of Electronic Publication: 2017 Aug 19.- Publication Type:
Clinical Trial, Phase III; Journal Article; Multicenter Study- Language:
English - Source:
- Additional Information
- Source: Publisher: Springer International Country of Publication: Germany NLM ID: 8708728 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-198X (Electronic) Linking ISSN: 0931041X NLM ISO Abbreviation: Pediatr Nephrol Subsets: MEDLINE
- Publication Information: Publication: Berlin : Springer International
Original Publication: Berlin : Springer International, c1987- - Subject Terms:
- Abstract: Background: In neonates, the validation of urinary biomarkers to diagnose acute kidney injury is a rapidly evolving field. The neonatal population poses unique challenges when assessing the collection, storage, and processing of urinary samples for biomarker analysis. Given this, establishing optimal and consistent sample processing in this population for meaningful use in ongoing clinical trials is important.
Methods: Urine from a cohort of 19 hospitalized neonatal intensive care unit patients enrolled in the Preterm Erythropoietin Neuroprotection Trial (Clinical Trial NCT01378273) was collected for biomarker analysis by indirect techniques using Fisher-brand cotton balls placed in the diapers. Fourteen urinary biomarkers were measured using commercially available kits via electrochemiluminescence on multiarray plates and compared between paired samples processed with centrifugation prior to storage versus prior to analysis.
Results: None of the biomarker concentrations differed between samples undergoing centrifugation prior to storage versus prior to analysis. The difference between samples was within 2% of the estimated concentration for the protein in 12 of 14 biomarkers (86%), and all paired biomarker concentrations were within 4%. The percentage error analysis did not show a difference between paired samples, with biomarker percentage errors smaller than the stated immunoassay coefficient of variance.
Conclusions: The urinary concentrations of biomarkers were comparable between paired samples, demonstrating that indirectly collected neonatal urine samples do not require centrifugation after collection and before storage. The ability to use routine urine collection and storage methods to obtain samples for subsequent quantitative immunoassay analysis should facilitate studies of newborns and young children. - References: Am J Kidney Dis. 2014 Apr;63(4):567-72. (PMID: 24200462)
J Clin Epidemiol. 2015 Aug;68(8):939-43. (PMID: 25895962)
Lancet. 1986 Feb 8;1(8476):307-10. (PMID: 2868172)
Am J Kidney Dis. 2016 Jan;67(1):56-61. (PMID: 26032628)
Clin J Am Soc Nephrol. 2016 Oct 7;11(10 ):1726-1728. (PMID: 27654929)
Lancet. 1995 Oct 21;346(8982):1085-7. (PMID: 7564793)
Clin Pediatr (Phila). 1991 May;30(5):278-80. (PMID: 2044335)
Am J Clin Nutr. 1992 Feb;55(2):326-30. (PMID: 1734668)
Clin J Am Soc Nephrol. 2016 Sep 7;11(9):1527-35. (PMID: 27471253)
J Pediatr. 2001 Dec;139(6):821-7. (PMID: 11743507)
Matern Health Neonatol Perinatol. 2015 Dec 02;1:27. (PMID: 27057344)
Clin Pediatr (Phila). 1993 May;32(5):314-5. (PMID: 8324981) - Grant Information: NCT01378273 National Institutes of Health; U01NS077953 National Institutes of Health; U54 HD083091 United States HD NICHD NIH HHS; DK103608 National Institutes of Health; T32DK007662 National Institutes of Health; T32 DK007662 United States DK NIDDK NIH HHS; R01 DK103608 United States DK NIDDK NIH HHS; U01 NS077953 United States NS NINDS NIH HHS
- Contributed Indexing: Keywords: Acute kidney injury*; Interleukin 18*; Kidney injury molecule 1*; Neonates*; Neutrophil gelatinase-associated lipocalin*; Urinary biomarkers*; Urine storage*
- Accession Number: 0 (Biomarkers)
- Publication Date: Date Created: 20170820 Date Completed: 20180618 Latest Revision: 20190718
- Publication Date: 20231215
- Accession Number: PMC5700848
- Accession Number: 10.1007/s00467-017-3779-0
- Accession Number: 28821985
- Source:
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