Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • Subject Terms:
      Actins/*chemistry ; Endothelium, Corneal/*pathology ; Ocular Hypertension/*pathology ; Sodium-Potassium-Exchanging ATPase/*metabolism ; Zonula Occludens-1 Protein/*chemistry; Actins/metabolism ; Animals ; Cell Count ; Cells, Cultured ; Dextrans/administration & dosage ; Disease Models, Animal ; Endothelial Cells/cytology ; Endothelial Cells/metabolism ; Endothelial Cells/pathology ; Endothelium, Corneal/cytology ; Endothelium, Corneal/metabolism ; Fluorescein-5-isothiocyanate/administration & dosage ; Fluorescein-5-isothiocyanate/analogs & derivatives ; Ocular Hypertension/etiology ; Ocular Hypertension/metabolism ; Permeability ; Rats ; Rats, Sprague-Dawley ; Zonula Occludens-1 Protein/metabolism
    • Abstract:
      Acute ocular hypertension (AOH) frequently compromises corneal endothelial cell (CEC) function in clinical practice. This type of stress induces corneal oedema and a decrease in the corneal endothelial cell density (ECD). The anterior chamber of the right eye of Sprague-Dawley rats was irrigated with Balanced Salt Solution (BSS) for two hours, and the left eye served as a control to determine the time-dependent effects of AOH on endothelial cell morphology and function. The average intraocular pressure (IOP) increased to 82.6 ± 2.3 mmHg (normal range: 10.2 ± 0.4 mmHg) during anterior irrigation. Very soon after initiating irrigation, corneal oedema became evident and the cornea exhibited a significant increase in permeability to FITC-dextran. The peripheral ECD was significantly reduced, and the morphology of CECs became irregular and multiform. The structures of the zonula occludens-1 (ZO-1) and F-actin were severely disrupted. In addtion, Na,K-ATPase exhibited a dispersed expression pattern. Two days after irrigation, obvious CEC proliferation was observed, the ECD recovered to a normal level, and F-actin was dispersed throughout the cytoplasm. Seven days later, the CEC structure and function were nearly normalized. Based on the results obtained using this model, an acute IOP crisis exerts transient deleterious effects on CEC structure and function in rats.
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    • Accession Number:
      0 (Actins)
      0 (Dextrans)
      0 (Zonula Occludens-1 Protein)
      0 (fluorescein isothiocyanate dextran)
      EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
      I223NX31W9 (Fluorescein-5-isothiocyanate)
    • Publication Date:
      Date Created: 20170802 Date Completed: 20190311 Latest Revision: 20191210
    • Publication Date:
      20221213
    • Accession Number:
      PMC5537405
    • Accession Number:
      10.1038/s41598-017-07534-9
    • Accession Number:
      28761172