Reconstruction of the microalga Nannochloropsis salina genome-scale metabolic model with applications to lipid production.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101301827 Publication Model: Electronic Cited Medium: Internet ISSN: 1752-0509 (Electronic) Linking ISSN: 17520509 NLM ISO Abbreviation: BMC Syst Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central
    • Subject Terms:
      Genomics* ; Models, Biological*; Lipids/*biosynthesis ; Microalgae/*genetics ; Microalgae/*metabolism ; Stramenopiles/*genetics ; Stramenopiles/*metabolism; Chromosome Mapping ; Gene Expression Profiling ; Genetic Engineering ; Molecular Sequence Annotation ; Nitrogen/metabolism
    • Abstract:
      Background: Nannochloropsis salina (= Eustigmatophyceae) is a marine microalga which has become a biotechnological target because of its high capacity to produce polyunsaturated fatty acids and triacylglycerols. It has been used as a source of biofuel, pigments and food supplements, like Omega 3. Only some Nannochloropsis species have been sequenced, but none of them benefit from a genome-scale metabolic model (GSMM), able to predict its metabolic capabilities.
      Results: We present iNS934, the first GSMM for N. salina, including 2345 reactions, 934 genes and an exhaustive description of lipid and nitrogen metabolism. iNS934 has a 90% of accuracy when making simple growth/no-growth predictions and has a 15% error rate in predicting growth rates in different experimental conditions. Moreover, iNS934 allowed us to propose 82 different knockout strategies for strain optimization of triacylglycerols.
      Conclusions: iNS934 provides a powerful tool for metabolic improvement, allowing predictions and simulations of N. salina metabolism under different media and genetic conditions. It also provides a systemic view of N. salina metabolism, potentially guiding research and providing context to -omics data.
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    • Contributed Indexing:
      Keywords: Genome-scale Metabolic model; Microalgæ; Nannochloropsis salina; TAG
    • Accession Number:
      0 (Lipids)
      N762921K75 (Nitrogen)
    • Publication Date:
      Date Created: 20170706 Date Completed: 20180312 Latest Revision: 20181113
    • Publication Date:
      20240829
    • Accession Number:
      PMC5496344
    • Accession Number:
      10.1186/s12918-017-0441-1
    • Accession Number:
      28676050