Direct measurement of IgM-Antigen interaction energy on individual red blood cells.

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  • Author(s): Yeow N;Yeow N; Tabor RF; Tabor RF; Garnier G; Garnier G
  • Source:
    Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2017 Jul 01; Vol. 155, pp. 373-378. Date of Electronic Publication: 2017 Apr 21.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam ; New York : Elsevier, c1993-
    • Subject Terms:
      Antigens/*immunology ; Erythrocytes/*immunology ; Immunoglobulin M/*immunology ; Single-Cell Analysis/*methods; Antigens/chemistry ; Antigens/metabolism ; Blood Grouping and Crossmatching/methods ; Energy Transfer ; Erythrocytes/metabolism ; Humans ; Immunoglobulin M/chemistry ; Immunoglobulin M/metabolism ; Microscopy, Atomic Force/methods ; Protein Binding
    • Abstract:
      Most blood grouping tests rely on the principle of red blood cells (RBCs) agglutination. Agglutination is triggered by the binding of specific blood grouping antibodies to the corresponding RBC surface antigen on multiple cells. The interaction energies between blood grouping antibodies and antigens have been poorly defined in immunohaematology. Here for the first time, we functionalized atomic force microscope (AFM) cantilevers with the IgM form of blood grouping antibodies to probe populations of individual RBCs of different groups under physiological conditions. The force-mapping mode of AFM allowed us to measure specific antibody - antigen interactions, and simultaneously localize and quantify antigen sites on the scanned cell surface. This study provides a new insight of the interactions between IgM antibodies and its corresponding antigen. The technique and information can be translated to develop better blood typing diagnostics and optimize target-specific drug delivery for medical applications.
      (Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Antibody; Antigen density; Atomic force microscope; Force mapping; IgM
    • Accession Number:
      0 (Antigens)
      0 (Immunoglobulin M)
    • Publication Date:
      Date Created: 20170429 Date Completed: 20180312 Latest Revision: 20180312
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.colsurfb.2017.04.038
    • Accession Number:
      28454066