Burkholderia cepacia complex in cystic fibrosis in the post-epidemic period: multilocus sequence typing-based approach.

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  • Author(s): Fila L;Fila L; Dřevínek P; Dřevínek P
  • Source:
    Folia microbiologica [Folia Microbiol (Praha)] 2017 Nov; Vol. 62 (6), pp. 509-514. Date of Electronic Publication: 2017 Mar 31.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: United States NLM ID: 0376757 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1874-9356 (Electronic) Linking ISSN: 00155632 NLM ISO Abbreviation: Folia Microbiol (Praha) Subsets: MEDLINE
    • Publication Information:
      Publication: New York : Springer
      Original Publication: Praha, Academia Scientiarum Bohemoslovenica.
    • Subject Terms:
      Burkholderia Infections/*microbiology ; Burkholderia cepacia complex/*isolation & purification ; Cystic Fibrosis/*microbiology; Burkholderia Infections/epidemiology ; Burkholderia cepacia complex/classification ; Burkholderia cepacia complex/genetics ; Cystic Fibrosis/epidemiology ; Czech Republic/epidemiology ; Female ; Humans ; Male ; Molecular Epidemiology ; Multilocus Sequence Typing ; Prevalence
    • Abstract:
      Cystic fibrosis (CF) patients in the Czech Republic suffered in the late 1990s from an epidemic with ST32 strain of Burkholderia cepacia complex (Bcc). Cohort segregation of Bcc and of ST32 positive patients was introduced in 1999 and 2002, respectively. We performed a study to evaluate the molecular epidemiology of Bcc infection after implementation of these infection control measures. Patients attending the Prague CF adult Centre from 2000 to 2015 were included in the present study. Demographic data and microbial statuses were collected from patient records. All Bcc isolates were analyzed using multilocus sequence typing (MLST). The prevalences of epidemic strain ST32 and of other Bcc strains were calculated. Ninety out of 227 CF patients were infected with Bcc during the study period. The prevalence of ST32 cases significantly decreased from 46.5% in 2000-2001 to 10.4% in 2014-2015 (P < 0.001) due to occurrence of only one new case in 2003, as well as to the death of 72% of ST32-infected patients. Conversely, there was a significant increase in prevalence of other Bcc strains, which rose from 0 to 14.9% (P = 0.015) and of transient infections. A micro-epidemic of infection with ST630 strain was observed in 2014 in lung transplant patients hospitalized in intensive care unit. The prevalence of epidemic strain ST32 decreased, whereas that of non-clonal strains of Bcc increased. Routine use of MLST allowed early detection of new and potentially epidemic strains.
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    • Contributed Indexing:
      Keywords: Cystic Fibrosis; Cystic Fibrosis Patient; Molecular Genetic Method; Multilocus Sequence Typing; recA Group
    • Publication Date:
      Date Created: 20170402 Date Completed: 20180514 Latest Revision: 20181113
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/s12223-017-0523-x
    • Accession Number:
      28364392