Characterization of Enterococcus species isolated from marine recreational waters by MALDI-TOF MS and Rapid ID API® 20 Strep system.

Publisher: Elsevier Country of Publication: England NLM ID: 0260231 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3363 (Electronic) Linking ISSN: 0025326X NLM ISO Abbreviation: Mar Pollut Bull Subsets: MEDLINE

Publication: 2003- : Oxford : Elsevier
Original Publication: London, Macmillan.

Bathing Beaches*; Enterococcus/*isolation & purification ; Seawater/*microbiology; Brazil ; Enterococcus/classification ; Humans ; RNA, Ribosomal, 16S/genetics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Water Microbiology

MALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioMérieux's API® 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods. The 16S rRNA gene sequencing of isolates with discrepant results showed that MALDI-TOF and API® correctly identified 74% and 11% of these isolates, respectively. This discrepancy probably relies on the bias of the API® has to identify clinical isolates. MALDI-TOF proved to be a feasible approach for identifying Enterococcus from environmental matrices increasing the rapidness and accuracy of results.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)

Keywords: 16S rRNA gene sequencing; Beaches; Enterococci; Gram-positive; MALDI-TOF MS; Species identification

0 (RNA, Ribosomal, 16S)

Date Created: 20170321 Date Completed: 20170524 Latest Revision: 20181202

20240829

10.1016/j.marpolbul.2017.03.025

28318563