Endophytic root bacteria associated with the natural vegetation growing at the hydrocarbon-contaminated Bitumount Provincial Historic site.

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  • Additional Information
    • Source:
      Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 0372707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1480-3275 (Electronic) Linking ISSN: 00084166 NLM ISO Abbreviation: Can J Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : Ottawa, ON : Canadian Science Publishing
      Original Publication: Ottawa, Canada : National Research Council, [1954-
    • Subject Terms:
      Hydrocarbons* ; Soil Microbiology* ; Soil Pollutants*/analysis; Plant Roots/*microbiology; Bacteria/genetics ; Biodegradation, Environmental ; Plant Development ; RNA, Bacterial ; RNA, Ribosomal, 16S ; Rhizosphere ; Soil/chemistry
    • Abstract:
      The Bitumount Provincial Historic site is the location of 2 of the world's first oil-extracting and -refining operations. Despite hydrocarbon levels ranging from 330 to 24 700 mg·(kg soil) -1 , plants have been able to recolonize the site through means of natural revegetation. This study was designed to achieve a better understanding of the plant-root-associated bacterial partnerships occurring within naturally revegetated hydrocarbon-contaminated soils. Root endophytic bacterial communities were characterized from representative plant species throughout the site by both high-throughput sequencing and culturing techniques. Population abundance of rhizosphere and root endosphere bacteria was significantly influenced (p < 0.05) by plant species and sampling location. In general, members of the Actinomycetales, Rhizobiales, Pseudomonadales, Burkholderiales, and Sphingomonadales orders were the most commonly identified orders. Community structure of root-associated bacteria was influenced by both plant species and sampling location. Quantitative real-time polymerase chain reaction was used to determine the potential functional diversity of the root endophytic bacteria. The gene copy numbers of 16S rRNA and 2 hydrocarbon-degrading genes (CYP153 and alkB) were significantly affected (p < 0.05) by the interaction of plant species and sampling location. Our findings suggest that some of the bacterial communities detected are known to exhibit plant growth promotion characteristics.
    • Contributed Indexing:
      Keywords: bactéries endophytes racinaires; communautés bactériennes; endophytic root bacteria; hydrocarbures pétroliers; microbial community; petroleum hydrocarbons; reclamation–remediation; réhabilitation–restauration
    • Accession Number:
      0 (Hydrocarbons)
      0 (RNA, Bacterial)
      0 (RNA, Ribosomal, 16S)
      0 (Soil)
      0 (Soil Pollutants)
    • Publication Date:
      Date Created: 20170225 Date Completed: 20171025 Latest Revision: 20181202
    • Publication Date:
      20231215
    • Accession Number:
      10.1139/cjm-2017-0039
    • Accession Number:
      28235184