Characterization of a Gallid herpesvirus 2 strain with novel reticuloendotheliosis virus long terminal repeat inserts.

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  • Additional Information
    • Source:
      Publisher: Kluwer Academic Country of Publication: United States NLM ID: 8803967 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1572-994X (Electronic) Linking ISSN: 09208569 NLM ISO Abbreviation: Virus Genes Subsets: MEDLINE
    • Publication Information:
      Publication: Boston Ma : Kluwer Academic
      Original Publication: Boston, USA : M. Nijhoff, 1987-
    • Subject Terms:
      Mutagenesis, Insertional*; Chickens/*virology ; Herpesvirus 2, Gallid/*genetics ; Marek Disease/*virology ; Reticuloendotheliosis virus/*genetics ; Terminal Repeat Sequences/*genetics; Animals ; China ; Coinfection/virology ; Disease Models, Animal ; Escherichia coli/genetics ; Genome, Viral ; Herpesvirus 2, Gallid/drug effects ; Herpesvirus 2, Gallid/immunology ; Marek Disease/immunology ; Marek Disease/mortality ; Marek Disease/prevention & control ; Oncogene Proteins, Viral/genetics ; Open Reading Frames ; Phylogeny ; Poultry Diseases/virology ; Sequence Analysis, DNA ; Vaccination ; Viral Vaccines ; Virulence ; Whole Genome Sequencing
    • Abstract:
      A bacterial artificial chromosome clone, designated LCY, was constructed from a Gallid herpesvirus 2 (GaHV-2) isolate from a GaHV-2 and reticuloendotheliosis virus co-infected clinical sample. The LCY GaHV-2 insert was sequenced and found to consist of 175,319 nucleotides. LCY GaHV-2 open reading frames (ORFs) had a high sequence identity to those of reference strains. The major difference was that two REV long terminal repeats (LTRs), in the same direction, were inserted at the internal repeat short (IRs)/unique short (Us) and Us/terminal repeat short (TRs) junctions. In addition, the a-like sequence and UL36 were different from other strains. Phylogenetic analysis revealed that LCY was closely related to pandemic strains in China. A pathogenicity study and a vaccination-challenge test were performed on LCY and the reference strain, GA. The results showed that LCY induced gross Marek's disease (MD) lesions and mortality in 71.4 and 7.1% of chickens, respectively, which are lower rates than those observed for the reference strain GA (85.7 and 35.7%). The commercially available CVI988 vaccine provided complete protection against LCY and GA (100%). These results showed that the isolate exhibited lower pathogenicity in SPF chickens. This study revealed that a novel pattern of LTR inserts was found in the strain LCY and that the strain was of low virulence. The present work expands the available genetic information for GaHV-2 and will be useful for the control of MD in China.
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    • Contributed Indexing:
      Keywords: Gallid herpesvirus 2; Genomic sequence; Molecular characterization; Pathogenicity; Reticuloendotheliosis virus
    • Accession Number:
      0 (Eco-Q protein, Gallid herpesvirus 2)
      0 (Oncogene Proteins, Viral)
      0 (Viral Vaccines)
    • Publication Date:
      Date Created: 20170215 Date Completed: 20180123 Latest Revision: 20201209
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s11262-017-1427-1
    • Accession Number:
      28194622